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Research Article

LC–MS/MS assay of ropinirole in rat biological matrices: elimination of lysoglycerophosphocholines-based matrix effect

    Xingjin Yan

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmacy, Chongqing General Hospital, Chongqing 400000, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmacy, Chongqing General Hospital, Chongqing 400000, PR China

    Authors contributed equally

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    ,
    Shouwei Zhao

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Authors contributed equally

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    ,
    Wenwen Zhu

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Jing Rao

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Li Kang

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Jiaqiang Xu

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Dan Li

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Lixia Qin

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Lihui Qiu

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Gao Li

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    ,
    Jiangeng Huang

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    &
    Luqin Si

    *Author for correspondence:

    E-mail Address: slq007@163.com

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China

    Published Online:https://doi.org/10.4155/bio-2016-0087

    Aim: To adequately support PK evaluation of ropinirole in rats following intranasal administration, it was desirable to determine ropinirole concentrations in rat plasma, brain homogenate and microdialysate. Results & methodology: A robust LC–MS/MS method has been developed for the determination of ropinirole in biological samples. Liquid–liquid extraction using ethyl acetate eliminated matrix effect due to significantly reduced levels of lysoglycerophosphocholines. The assay was fully validated with dynamic ranges of 0.01–20 ng/ml for plasma and brain homogenate samples and 0.1–200 ng/ml for microdialysate samples. Conclusion: The proposed method was accurate and precise for the quantification of ropinirole in biological samples and was successfully applied to a microdialysis study of ropinirole in rats.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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