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Extraction of Nuclear Proteins with Increased DNA Binding Activity

    Beatrix A. Slomiany

    Medical University of South Carolina, Charleston, SC, USA

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    ,
    Margaret M. Kelly

    Medical University of South Carolina, Charleston, SC, USA

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    &
    David T. Kurtz

    *Address correspondence to Dr. David T. Kurtz, Department of Pharmacology, 173 Ashley Ave., P.O. Box 250505, Charleston, SC 29425, USA. Internet:

    E-mail Address: kurtzdt@musc.edu

    Medical University of South Carolina, Charleston, SC, USA

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    Published Online:29 Aug 2018https://doi.org/10.2144/00285st08

    Abstract

    We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number of samples for gel shift analysis.