BioTechniquesVol. 29, No. 4 BenchmarksOpen AccessIncreased Sensitivity of One-Tube, Quantitative RT-PCRS. Raja, J.D. Luketich, L.A. Kelly, D.W. Ruff & T.E. GodfreyS. RajaUniversity of Pittsburgh Medical Center, Pittsburgh, PASearch for more papers by this author, J.D. LuketichUniversity of Pittsburgh Medical Center, Pittsburgh, PASearch for more papers by this author, L.A. KellyUniversity of Pittsburgh Medical Center, Pittsburgh, PASearch for more papers by this author, D.W. RuffUniversity of Pittsburgh Medical Center, Pittsburgh, PASearch for more papers by this author & T.E. Godfrey*Address correspondence to Dr. Tony E. Godfrey, C800, Presbyterian University Hospital, 200 Lothrop Street, Pittsburgh, PA 15213, USA. e-mail: E-mail Address: godfreyte@msx.upmc.eduUniversity of Pittsburgh Medical Center, Pittsburgh, PASearch for more papers by this authorPublished Online:29 Aug 2018https://doi.org/10.2144/00294bm06AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByTransgene mRNA Expression Analysis16 February 2024Polymerase Chain Reaction: Applications in Gene and Cell Therapy Studies5 April 2023MOL-PCR and xMAP TechnologyThe Journal of Molecular Diagnostics, Vol. 23, No. 6Marker Development for Important Grapevine Traits by Genetic Diversity Studies and Investigation of Differential Gene Expression2 August 2010Comparison of ‘real-time’ and immunometric RT-PCR: RNA interference of reverse transcriptase-PCRJournal of Immunological Methods, Vol. 312, No. 1-2Identification of mRNA Markers for Molecular Staging of Lymph Nodes in Colorectal Cancer1 March 2006 | Clinical Chemistry, Vol. 52, No. 3The Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes Are Not Expressed in the Rat HeartEndocrinology, Vol. 146, No. 12Technology for Automated, Rapid, and Quantitative PCR or Reverse Transcription-PCR Clinical Testing1 May 2005 | Clinical Chemistry, Vol. 51, No. 5Characterization of Amplifiable, Circulating RNA in Plasma and Its Potential as a Tool for Cancer Diagnostics1 March 2004 | Clinical Chemistry, Vol. 50, No. 3Temperature-controlled Primer Limit for Multiplexing of Rapid, Quantitative Reverse Transcription-PCR Assays: Application to Intraoperative Cancer Diagnostics1 August 2002 | Clinical Chemistry, Vol. 48, No. 8Rapid, quantitative reverse transcriptase-polymerase chain reaction: Application to intraoperative molecular detection of occult metastases in esophageal cancerThe Journal of Thoracic and Cardiovascular Surgery, Vol. 123, No. 3 Vol. 29, No. 4 STAY CONNECTED Metrics Downloaded 384 times History Published online 29 August 2018 Published in print October 2000 Information© 2018 Author(s)PDF download