BioTechniquesVol. 33, No. 2 Short Technical ReportsOpen AccessOptimizing DpnI Digestion Conditions to Detect Replicated DNALu Lu, Hiren Patel & John J. BisslerLu LuThe Children’s Hospital Research Foundation, Cincinnati, OH, USASearch for more papers by this author, Hiren PatelThe Children’s Hospital Research Foundation, Cincinnati, OH, USASearch for more papers by this author & John J. Bissler*Address correspondence to Dr. John J. Bissler, Children’s Hospital Research Foundation, R5, 3333 Burnet Ave., Cincinnati, OH 45229-3039, USA. e-mail: E-mail Address: john.bissler@chmcc.orgThe Children’s Hospital Research Foundation, Cincinnati, OH, USASearch for more papers by this authorPublished Online:17 Sep 2018https://doi.org/10.2144/02332st03AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited BySite-Directed Mutagenesis to Mutate Multiple Residues in a Single Reaction6 January 2024Development of a compartmentalised self-replication protocol for selection of superior blunt-end DNA ligasesEnzyme and Microbial Technology, Vol. 163Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmidsNew Biotechnology, Vol. 72Z-DNA as a Tool for Nuclease-Free DNA Methyltransferase Assay5 November 2021 | International Journal of Molecular Sciences, Vol. 22, No. 21Crystal structure of the modification-dependent SRA-HNH endonuclease TagI7 September 2018 | Nucleic Acids Research, Vol. 455Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila13 September 2017Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses20 June 2016 | Chemical Reviews, Vol. 116, No. 20Absence of MutSβ leads to the formation of slipped-DNA for CTG/CAG contractions at primate replication forksDNA Repair, Vol. 42Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression6 February 2016 | Journal of Zhejiang University-SCIENCE B, Vol. 17, No. 2Structural basis of the methylation specificity of R.DpnI25 June 2014 | Nucleic Acids Research, Vol. 42, No. 13The other face of restriction: modification-dependent enzymes29 August 2013 | Nucleic Acids Research, Vol. 42, No. 1SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites22 March 2013 | BMC Bioinformatics, Vol. 14, No. 1Substrate specificity and properties of methyl-directed site-specific DNA endonucleases10 December 2013 | Molecular Biology, Vol. 47, No. 6Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirusVirus Research, Vol. 175, No. 1Crystal structure and mechanism of action of the N6-methyladenine-dependent type IIM restriction endonuclease R.DpnI5 June 2012 | Nucleic Acids Research, Vol. 40, No. 15Replication efficiency of rolling-circle replicon-based plasmids derived from porcine circovirus 2 in eukaryotic cellsJournal of Virological Methods, Vol. 165, No. 1Site-Directed Mutagenesis of Whole Viral GenomesA site-directed mutagenesis method utilising large double-stranded DNA templates for the simultaneous introduction of multiple changes and sequential multiple rounds of mutation: Application to the study of whole viral genomesJournal of Virological Methods, Vol. 137, No. 1Methylation Sensitivity of Restriction Enzymes Interacting with GATC SitesAndrea Hermann & Albert Jeltsch24 September 2018 | BioTechniques, Vol. 34, No. 5Unpaired Structures in SCA10 (ATTCT)n·(AGAAT)n RepeatsJournal of Molecular Biology, Vol. 326, No. 4 Vol. 33, No. 2 STAY CONNECTED Metrics Downloaded 2,439 times History Published online 17 September 2018 Published in print August 2002 Information© 2018 Author(s)PDF download