BioTechniquesVol. 35, No. 4 BenchmarksOpen AccessComplex DNA melting profiles of small PCR products revealed using SYBR® Green IWeijie Li, Biao Xi, Wancai Yang, Meredith Hawkins & Ulrich K. SchubartWeijie LiAlbert Einstein College of Medicine, Bronx, NY, USASearch for more papers by this author, Biao XiAlbert Einstein College of Medicine, Bronx, NY, USASearch for more papers by this author, Wancai YangAlbert Einstein College of Medicine, Bronx, NY, USASearch for more papers by this author, Meredith HawkinsAlbert Einstein College of Medicine, Bronx, NY, USASearch for more papers by this author & Ulrich K. Schubart*Address correspondence to Ulrich K. Schubart, Department of Medicine, Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. e-mail: E-mail Address: schubart@aecom.yu.eduAlbert Einstein College of Medicine, Bronx, NY, USASearch for more papers by this authorPublished Online:24 Sep 2018https://doi.org/10.2144/03354bm07AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByMolecular detection and identification of Diatrypaceous airborne spores in Australian vineyards revealed high species diversity between regions2 June 2023 | PLOS ONE, Vol. 18, No. 6Synergistic Antimicrobial Effect of UV-A Irradiation and Malic Acid Combination Treatment Against Foodborne Pathogens on Spinach and the Underlying Mechanism19 January 2022 | Food and Bioprocess Technology, Vol. 15, No. 2Continuous Pest Surveillance and Monitoring Constitute a Tool for Sustainable Agriculture: Case of Xylella fastidiosa in Morocco27 January 2022 | Sustainability, Vol. 14, No. 3Growth temperature influences the resistance of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium on lettuce to X-ray irradiationFood Microbiology, Vol. 99GLADS: A gel-less approach for detection of STMS markers in wheat and rice5 November 2019 | PLOS ONE, Vol. 14, No. 11A New Method for Rapid Screening of End-Point PCR Products: Application to Single Genome Amplified HIV and SIV Envelope Amplicons8 June 2015 | PLOS ONE, Vol. 10, No. 6Molecular characterisation of ABC-type multidrug efflux systems in Bifidobacterium longumAnaerobe, Vol. 32Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters3 October 2014 | Journal of Applied Microbiology, Vol. 117, No. 5Quantification of Pythium populations in ginseng soils by culture dependent and real-time PCR methodsApplied Soil Ecology, Vol. 40, No. 3A study of comparability in amplified fragment length polymorphism profiling using a simple model system14 September 2007 | ELECTROPHORESIS, Vol. 28, No. 18Characterization of rsmC as a potential reference gene for Salmonella Typhimurium gene expression during growth in spent media15 June 2007 | Sensing and Instrumentation for Food Quality and Safety, Vol. 1, No. 3AUTOMATED VALIDATION OF POLYMERASE CHAIN REACTION AMPLICON MELTING CURVES21 November 2011 | Journal of Bioinformatics and Computational Biology, Vol. 04, No. 02In situ monitoring of polymerase extension rate and adaptive feedback control of PCR by using fluorescence measurementsJournal of Biochemical and Biophysical Methods, Vol. 65, No. 2-3Antennal expressed genes of the yellow fever mosquito (Aedes aegypti L.); characterization of odorant-binding protein 10 and takeoutInsect Biochemistry and Molecular Biology, Vol. 35, No. 9A Duplex Real-Time qPCR Assay for the Quantification of Human Nuclear and Mitochondrial DNA in Forensic Samples: Implications for Quantifying DNA in Degraded SamplesJournal of Forensic Sciences, Vol. 50, No. 5Differentiation between amplicon polymerization and nonspecific products in SYBR green I real-time polymerase chain reactionAnalytical Biochemistry, Vol. 342, No. 1Alternatives to antibiotics: chemical and physical antimicrobial interventions and foodborne pathogen responsePoultry Science, Vol. 84, No. 4Automated validation of polymerase chain reactions using amplicon melting curvesComparison of primers for the detection of Salmonella enterica serovars using real-time PCRLetters in Applied Microbiology, Vol. 39, No. 2 Vol. 35, No. 4 STAY CONNECTED Metrics Downloaded 249 times History Published online 24 September 2018 Published in print October 2003 Information© 2018 Author(s)PDF download