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Research Article

Enantiomeric analysis of clenbuterol in Chinese people by LC–MS/MS to distinguish doping abuse from meat contamination

    Genye He‡

    National Anti-Doping Laboratory, China Anti-Doping Agency, 1 Anding Road, Chaoyang District, Beijing 100029, PR China

    ‡Authors contributed equally

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    ,
    Linghui Sheng‡

    National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital Capital Medical University, Beijing 100053, PR China

    ‡Authors contributed equally

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    ,
    Jianli Zhang

    China Institute of Sport Science, No.11 Stadium Road, Dongcheng District, Beijing 100061, PR China

    ,
    Yun Wu

    National Anti-Doping Laboratory, China Anti-Doping Agency, 1 Anding Road, Chaoyang District, Beijing 100029, PR China

    ,
    Xuxiao Zhao

    Drug & Food Anti-Doping laboratory, China Anti-Doping Agency, 1st Anding Road, Chaoyang District, Beijing 100029, PR China

    ,
    Youxuan Xu

    **Author for correspondence: Tel.: +86 106 489 8046; Fax: +86 106 489 8046;

    E-mail Address: xuyouxuan@chinada.cn

    Drug & Food Anti-Doping laboratory, China Anti-Doping Agency, 1st Anding Road, Chaoyang District, Beijing 100029, PR China

    &
    Jianghai Lu

    *Author for correspondence: Tel.: +86 106 489 8046; Fax: +86 106 489 8046;

    E-mail Address: lujianghai@chinada.cn

    Drug & Food Anti-Doping laboratory, China Anti-Doping Agency, 1st Anding Road, Chaoyang District, Beijing 100029, PR China

    Published Online:https://doi.org/10.4155/bio-2020-0003

    Aim: Follow-up investigations are often required for clenbuterol-positive cases. A method to distinguish doping abuse from meat contamination was developed. Materials & methods: A total of 26 volunteers were recruited to ingest clenbuterol contaminated-pork and clenbuterol tablets. Results: For 20 volunteers, after ingestion of contaminated-pork, R-(-)/S-(+)-clenbuterol ratio was <1.0, while the value was >1.0 after taking clenbuterol tablets. However, after taking clenbuterol tablets, some ratio points of the other six volunteers were between 0.9 and 1.0. A case of an abnormal cold and fever, which returned to normal after recovery, was also reported firstly. Conclusion: A change in R-(-)/S-(+)-clenbuterol was reported in the Chinese population initially. A ratio of 0.9 was recommended in doping related cases for the Chinese population.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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