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Simultaneous determination of canrenone, digoxin and tolvaptan by UHPLC–MS/MS: application in heart failure patients

    Peng Guo

    Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou City, Guangdong Province 510006, PR China

    ,
    Qiucheng Liang

    Cardiovascular Department of The First Affiliated Hospital of Guangzhou Medical University, Guangzhou City, Guangdong Province 510120, PR China

    ,
    Juntao Zheng

    Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou City, Guangdong Province 510006, PR China

    ,
    Jinmin Chen

    Cardiovascular Department of The First Affiliated Hospital of Guangzhou Medical University, Guangzhou City, Guangdong Province 510120, PR China

    ,
    Yanping Guan

    Department of Pharmacy, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou City, Guangdong Province 510120, PR China

    ,
    Liang Ding

    Clinical Trial & Research Center, People's Hospital of Shenzhen Baoan District, Shenzhen City, Guangdong Province 518101, PR China

    ,
    Fulin Jiang

    Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou City, Guangdong Province 510006, PR China

    ,
    Xuewen Chen

    Cardiovascular Department of The First Affiliated Hospital of Guangzhou Medical University, Guangzhou City, Guangdong Province 510120, PR China

    ,
    Min Huang

    Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou City, Guangdong Province 510006, PR China

    ,
    Ailan Chen

    *Authors for correspondence: Tel.: (020) 87335429-113; Fax: (020) 87335429-113;

    E-mail Address: zhonggp@mail.sysu.edu.cn

    Cardiovascular Department of The First Affiliated Hospital of Guangzhou Medical University, Guangzhou City, Guangdong Province 510120, PR China

    &
    Guoping Zhong

    *Authors for correspondence: Tel.: (020) 87335429-113; Fax: (020) 87335429-113;

    E-mail Address: zhonggp@mail.sysu.edu.cn

    Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou City, Guangdong Province 510006, PR China

    Published Online:https://doi.org/10.4155/bio-2020-0037

    Aim: Heart failure patients are frequently given comedication of digoxin and diuretics like spironolactone and tolvaptan. A UHPLC–MS/MS assay for determining canrenone (main active metabolite of spironolactone), digoxin and tolvaptan simultaneously should be developed so as to support related drug–drug interaction studies. Results: A UHPLC–MS/MS method for simultaneous determination of these three drugs in human plasma was established and fully verified as per CFDA guidelines. Chromatographic separation was achieved using a 4-min isocratic elution. Mass analyses were performed under positive electrospray ionization mode. The calibration curves were established over 1.0–400.0 ng/ml for canrenone and tolvaptan while over 0.1–40.0 ng/ml for digoxin. Conclusion: The developed method was feasible in detecting concentration and related drug–drug interaction studies.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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