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The future key role of LC–high-resolution-MS analyses in clinical laboratories: a focus on quantification

    Bertrand Rochat

    * Author for correspondence

    Quantitative MS Facility, University Hospital of Lausanne, CHUV, 1011 Lausanne, Switzerland.

    ,
    Emmanuel Kottelat

    Quantitative MS Facility, University Hospital of Lausanne, CHUV, 1011 Lausanne, Switzerland

    &
    Justin McMullen

    Quantitative MS Facility, University Hospital of Lausanne, CHUV, 1011 Lausanne, Switzerland

    Published Online:https://doi.org/10.4155/bio.12.243

    For the last decade, high-resolution (HR)-MS has been associated with qualitative analyses while triple quadrupole MS has been associated with routine quantitative analyses. However, a shift of this paradigm is taking place: quantitative and qualitative analyses will be increasingly performed by HR-MS, and it will become the common ‘language’ for most mass spectrometrists. Most analyses will be performed by full-scan acquisitions recording ‘all’ ions entering the HR-MS with subsequent construction of narrow-width extracted-ion chromatograms. Ions will be available for absolute quantification, profiling and data mining. In parallel to quantification, metabotyping will be the next step in clinical LC–MS analyses because it should help in personalized medicine. This article is aimed to help analytical chemists who perform targeted quantitative acquisitions with triple quadrupole MS make the transition to quantitative and qualitative analyses using HR-MS. Guidelines for the acceptance criteria of mass accuracy and for the determination of mass extraction windows in quantitative analyses are proposed.

    Papers of special note have been highlighted as: ▪ of interest

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