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Research Article

A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper

    Gabriel Lenk

    *Author for correspondence:

    E-mail Address: glenk@kth.se

    Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden

    ,
    Sören Sandkvist

    Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden

    ,
    Anton Pohanka

    Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden

    ,
    Göran Stemme

    Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden

    ,
    Olof Beck

    Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden

    &
    Niclas Roxhed

    Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden

    Published Online:https://doi.org/10.4155/bio.15.134

    Background: DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult. Results: We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 µl ± 7.1% (n = 21) for deionized water and 6.1 µl ± 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper. Conclusion: The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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