Abstract
Hydrophilic interaction chromatography (HILIC) is being increasingly used for the analysis of hydrophilic compounds in biological matrices. The complexity of biological samples demands adequate sample preparation procedures, specifically adjusted for HILIC analyses. Currently, most bioanalytical assays are performed on bare silica and ZIC-HILIC columns. Trends in HILIC for bioanalysis include smaller particle sizes and miniaturization of the analytical column. For complex biological samples, multidimensional techniques can separate and identify more compounds than 1D separations. The high volatility of the mobile phase, the added separation power and high sensitivity make MS the detection method of choice for bioanalysis using HILIC, although other detectors such as evaporative light scattering detection, charged aerosol detection and nuclear magnetic resonance have been reported.
References
- 1 . Bioequivalence of Sandoz methylphenidate osmotic-controlled release tablet with Concerta ® (Janssen-Cilag). Pharmacol. Res. Perspect. 3(1), 1–8 (2015).
- 2 Pharmacokinetic properties of MH84, a γ-secretase modulator with PPARγ agonistic activity. J. Pharm. Biomed. Anal. 102, 417–424 (2015).
- 3 . Microfluidic sample preparation for diagnostic cytopathology. Lab Chip. 13(6), 1011–1026 (2013).
- 4 . Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC–MS/MS. Anal. Bioanal. Chem. 407(13), 3647–3658 (2015).
- 5 Screening for anabolic steroids in sports: analytical strategy based on the detection of phase I and phase II intact urinary metabolites by liquid chromatography tandem mass spectrometry. J. Chromatogr. A. 1389, 65–75 (2015).
- 6 . Liquid chromatography of saccharides. J. Chromatogr. A. 105, 125–133 (1975).
- 7 . Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J. Chromatogr. 499, 177–196 (1990).
- 8 . Hydrophilic interaction liquid chromatography (HILIC)-a powerful separation technique. Anal. Bioanal. Chem. 402(1), 231–247 (2012).
- 9 . Simultaneous determination of nicotinic acid and its metabolites using hydrophilic interaction chromatography with tandem mass spectrometry. Rapid Commun. Mass Spectrom. 19(21), 3031–3036 (2005).
- 10 . Hydrophilic interaction chromatography using amino and silica columns for the determination of polar pharmaceuticals and impurities. J. Chromatogr. A. 913(1–2), 113–122 (2001).
- 11 Simultaneous determination of 15 aminoglycoside(s) residues in animal derived foods by automated solid-phase extraction and liquid chromatography-tandem mass spectrometry. Food Chem. 135(2), 676–683 (2012).
- 12 Determination of cocaine/crack biomarkers in colostrum by LC–MS following protein precipitation. J. Pharm. Biomed. Anal. 103, 67–72 (2015).
- 13 . Amino acid analysis by hydrophilic interaction chromatography coupled on-line to electrospray ionization mass spectrometry. Amino Acids 30(3), 291–297 (2006).
- 14 . Evaluation and optimization of ZIC-HILIC-RP as an alternative MudPIT strategy. J. Proteome Res. 6(3), 937–946 (2007).
- 15 . Hydrophilic interaction liquid chromatography in food analysis. J. Chromatogr. A. 1218(42), 7438–7452 (2011).
- 16 . Molecular dynamics simulation of water-acetonitrile mixtures in a silica slit. J. Phys. Chem. C. 117(8), 3923–3929 (2013).
- 17 . A molecular dynamics study on the partitioning mechanism in hydrophilic interaction chromatography. Angew. Chemie. Int. Ed. Engl. 51(25), 6251–6254 (2012).
- 18 . Hydrophilic interaction chromatography. J. Sep. Sci. 29(12), 1784–1821 (2006).
- 19 . Stationary and mobile phases in hydrophilic interaction chromatography: a review. Anal. Chim. Acta 692(1–2), 1–25 (2011).
- 20 . Retention and selectivity of stationary phases for hydrophilic interaction chromatography. J. Chromatogr. A. 1218(35), 5920–5938 (2011).
- 21 . The relative importance of the adsorption and partitioning mechanisms in hydrophilic interaction liquid chromatography. J. Chromatogr. A. 1376, 112–25 (2015).
- 22 Ultrafast liquid chromatography/tandem mass spectrometry bioanalysis of polar analytes using packed silica columns. Rapid Commun. Mass Spectrom. 16(17), 1613–1621 (2002).
- 23 . Hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics. J. Pharm. Biomed. Anal. 87, 142–154 (2014).
- 24 . Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds? J. Chromatogr. A. 1171(1–2), 46–55 (2007).
- 25 Fingerprint analysis of Ligusticum chuanxiong using hydrophilic interaction chromatography and reversed-phase liquid chromatography. J Chromatogr. A. 1216, 2136–2141 (2009).
- 26 . Orthogonality of separation in two-dimensional liquid chromatography. Anal. Chem. 77(19), 6426–6434 (2005).
- 27 . Evaluation of hydrophilic interaction chromatography (HILIC) versus C18 reversed-phase chromatography for targeted quantification of peptides by mass spectrometry. J. Chromatogr. A. 1264, 31–39 (2012).
- 28 . Systematic comparison of sensitivity between hydrophilic interaction liquid chromatography and reversed phase liquid chromatography coupled with mass spectrometry. J. Chromatogr. A. 1312, 49–57 (2013).
- 29 . Recent advances in application of hydrophilic interaction chromatography for quantitative bioanalysis. J. Sep. Sci. 33(6–7), 681–697 (2010).
- 30 . Recent advances in high-throughput quantitative bioanalysis by LC–MS/MS. J. Pharm. Biomed. Anal. 44(2), 342–355 (2007).
- 31 . A systematic investigation of the effect of sample diluent on peak shape in hydrophilic interaction liquid chromatography. J. Chromatogr. A. 1217(52), 8230–8240 (2010).
- 32 . A one-year monitoring of nicotine use in sport: frontier between potential performance enhancement and addiction issues. Forensic Sci. Int. 213(1–3), 73–84 (2011).
- 33 . An improved LC–MS/MS method for the determination of taspoglutide in plasma and urine using orthogonal HILIC-RP column switching, ultra-performance LC separation and “wrong-way-round” electrospray ionization. Biomed. Chromatogr. 25(11), 1215–1221 (2011).
- 34 A new HILIC-MS/MS method for the simultaneous analysis of carbidopa, levodopa, and its metabolites in human plasma. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 967, 41–49 (2014).
- 35 Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the simultaneous determination of five first-line antituberculosis drugs in plasma. Anal. Bioanal. Chem. 405(19), 6323–6335 (2013).
- 36 . Quantification of cisplatin, carboplatin and oxaliplatin in spiked human plasma samples by ICP-SFMS and hydrophilic interaction liquid chromatography (HILIC) combined with ICP-MS detection. J. Anal. At. Spectrom. 24(10), 1336–1342 (2009).
- 37 Determination and validation of tetrodotoxin in human whole blood using hydrophilic interaction liquid chromatography-tandem mass spectroscopy and its application. Forensic Sci. Int. 217(1–3), 76–80 (2012).
- 38 . Bioanalysis of acetylcarnitine in cerebrospinal fluid by HILIC-mass spectrometry. Biomed. Chromatogr. 29, 1375–1379 (2015).
- 39 . Determination of metformin in human plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 877(29), 3695–3700 (2009).
- 40 . Determination of zanamivir in rat and monkey plasma by positive ion hydrophilic interaction chromatography (HILIC)/tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 852(1–2), 505–511 (2007).
- 41 . Determination of sitagliptinin human plasma using protein precipitation and tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 878(21), 1817–1823 (2010).
- 42 . Comparison of hydrophilic interaction and reversed-phase liquid chromatography coupled with tandem mass spectrometric detection for the determination of three pharmaceuticals in human plasma. Biomed. Chromatogr. 22(10), 1139–1402 (2008).
- 43 . Determination of oral bioavailability of fusaric acid in male Sprague-Dawley rats. Drugs R. D. 14(2), 139–145 (2014).
- 44 . HybridSPE: a novel technique to reduce phospholipid-based matrix effect in LC-ESI-MS Bioanalysis. J. Pharm. Bioallied Sci. 4(4), 267–275 (2012).
- 45 . Liquid/liquid extraction using 96-well plate format in conjunction with hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the analysis of fluconazole in human plasma. J. Pharm. Biomed. Anal. 31(5), 917–928 (2003).
- 46 . Analysis of omeprazole and 5-OH omeprazole in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry (HILIC-MS/MS) - Eliminating evaporation and reconstitution steps in 96-well liquid/liquid extraction. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 830(1), 135–142 (2006).
- 47 . A 96-well single-pot liquid-liquid extraction, hydrophilic interaction liquid chromatography-mass spectrometry method for the determination of muraglitazar in human plasma. J. Pharm. Biomed. Anal. 41(3), 979–988 (2006).
- 48 . Libraries of N-alkylaminoheterocycles from nucleophilic aromatic substitution with purification by solid supported liquid extraction. Tetrahedron 54(16), 4097–4106 (1998).
- 49 . Automatic supported liquid extraction (SLE) coupled with HILIC-MS/MS: an application to method development and validation of erlotinib in human plasma. Pharmaceutics 2(2), 105–118 (2010).
- 50 . Direct injection HILIC–MS/MS analysis of darunavir in rat plasma applying supported liquid extraction. J. Pharm. Anal. 5(1), 43–50 (2015).
- 51 . UHPLC in modern bioanalysis. In: UHPLC in Life Sciences. Guillarme D, Veuthey JL (Eds). Royal Society of Chemistry, Cambridge, UK, 237–277 (2012).
- 52 . Quantification of nerve agent biomarkers in human serum and urine. Anal. Chem. 86, 11833–11840 (2014).
- 53 . Performance characteristics of an LC–MS/MS method for the determination of plasma metanephrines. Clin. Chim. Acta 413(19–20), 1459–1465 (2012).
- 54 Automated 96-well solid phase extraction and hydrophilic interaction liquid chromatography–tandem mass spectrometric method for the analysis of cetirizine (ZYRTEC) in human plasma - With emphasis on method ruggedness. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 814(1), 105–114 (2005).
- 55 . Development and validation of a liquid chromatographic-tandem mass spectrometric method for determination of oseltamivir and its metabolite oseltamivir carboxylate in plasma, saliva and urine. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 859(1), 74–83 (2007).
- 56 . Quantitative measurement of propofol and in main glucuroconjugate metabolites in human plasma using solid phase extraction-liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 854(1–2), 165–172 (2007).
- 57 . On-line solid phase extraction-liquid chromatography, with emphasis on modern bioanalysis and miniaturized systems. J. Pharm. Biomed. Anal. 87, 120–129 (2014).
- 58 Improving the resolution of neuropeptides in rat brain with on-line HILIC-RP compared with on-line SCX-RP. J. Sep. Sci. 31(3), 459–467 (2008).
- 59 . Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE-HILIC-MS/MS. J. Chromatogr. A. 1217(28), 4688–4695 (2010).
- 60 . Rapid and sensitive HILIC-ESI-MS/MS quantitation of polar metabolites of acrylamide in human urine using column switching with an online trap column. J. Agric. Food Chem. 56(21), 9828–9834 (2008).
- 61 . Liquid chromatography-mass spectrometry platform for both small neurotransmitters and neuropeptides in blood, with automatic and robust solid phase extraction. Sci. Rep. 5, 9308 (2015).
- 62 . How to address the sample preparation of hydrophilic compounds: determination of entecavir in plasma and plasma ultrafiltrate with novel extraction sorbents. J. Pharm. Biomed. Anal. 88, 377–344 (2014).
- 63 . Recent advances in microextraction by packed sorbent for bioanalysis. J. Chromatogr. A. 1217(16), 2569–2580 (2010).
- 64 . New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications: part III: determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS-LC–MS-MS. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 813(1–2), 129–135 (2004).
- 65 . Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography–ultra-high-performance liquid chromatography–tandem mass spectrometry. J. Pharm. Biomed. Anal. 109, 171–176 (2015).
- 66 . Systematic evaluation of solid-phase microextraction coatings for untargeted metabolomic profiling of biological fluids by liquid chromatography-mass spectrometry. Anal. Chem. 83(6), 1944–1954 (2011).
- 67 Semi-automated in vivo solid-phase microextraction sampling and the diffusion-based interface calibration model to determine the pharmacokinetics of methoxyfenoterol and fenoterol in rats. Anal. Chim. Acta 742, 37–44 (2012).
- 68 . Direct analysis of dried blood spots by in-line desorption combined with high-resolution chromatography and mass spectrometry for quantification of maple syrup urine disease biomarkers leucine and isoleucine. Anal. Bioanal. Chem. 400(1), 237–244 (2011).
- 69 . Polymer monolith microextraction online coupled to hydrophilic interaction chromatography/mass spectrometry for analysis of β2-agonist in human urine. J. Sep. Sci. 32(11), 1965–1974 (2009).
- 70 . Development of a molecularly imprinted polymer for selective extraction followed by liquid chromatographic determination of sitagliptin in rat plasma and urine. Talanta 85(2), 950–957 (2011).
- 71 Hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) determination of cocaine and its metabolites benzoylecgonine, ecgonine methyl ester, and cocaethylene in hair samples. Anal. Bioanal. Chem. 396(5), 1703–1712 (2010).
- 72 . Evaluation of different column chemistries for fast urinary metabolic profiling. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 927, 90–96 (2013).
- 73 HILIC LC–MS for the determination of 2′-C-methyl-cytidine-triphosphate in rat liver. J. Sep. Sci. 32(9), 1275–1283 (2009).
- 74 . Development of analytical method for catechol compounds in mouse urine using hydrophilic interaction liquid chromatography with fluorescence detection. J. Chromatogr. B. 985, 142–148 (2015).
- 75 . Highly sensitive fast determination of entecavir in rat urine by means of hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. A. 1259, 237–243 (2012).
- 76 . Alkaline conditions in hydrophilic interaction liquid chromatography for intracellular metabolite quantification using tandem mass spectrometry. Anal. Biochem. 475, 4–13 (2015).
- 77 . Sample preparation and UHPLC-FD analysis of pteridines in human urine. J. Pharm. Biomed. Anal. 95, 265–272 (2014).
- 78 . Estimation of the extent of the water-rich layer associated with the silica surface in hydrophilic interaction chromatography. J. Chromatogr. A. 1192(2), 225–229 (2008).
- 79 High-throughput hydrophilic interaction chromatography coupled to tandem mass spectrometry for the optimized quantification of the anti-Gram-negatives antibiotic colistin A/B and its pro-drug colistimethate. J. Chromatogr. A. 1369, 52–63 (2014).
- 80 . Hydrophilic interaction chromatography of polar and ionizable compounds by UHPLC. TrAC Trends Anal. Chem. 63, 55–64 (2014).
- 81 Strategies for quantitation of endogenous adenine nucleotides in human plasma using novel ion-pair hydrophilic interaction chromatography coupled with tandem mass spectrometry. J. Chromatogr. A. 1325, 129–136 (2014).
- 82 . Simplified cysteine dioxygenase activity assay allows simultaneous quantitation of both substrate and product. Anal. Biochem. 405(1), 127–131 (2010).
- 83 . Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC–MS. Anal. Bioanal. Chem. 406(2), 401–407 (2014).
- 84 . Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 878(31), 3303–3316 (2010).
- 85 . Evaluation of various HILIC materials for the fast separation of polar compounds. J. Sep. Sci. 33(6–7), 752–764 (2010).
- 86 . Development of a UHPLC–MS/MS method for the determination of plasma histamine in various mammalian species. J. Chromatogr. B. 971, 35–42 (2014).
- 87 Determination of N-methylcytisine in rat plasma by UPLC–MS/MS and its application to pharmacokinetic study. J. Chromatogr. B. 990, 118–124 (2015).
- 88 . Kinetic performance comparison of fully and superficially porous particles with a particle size of 5 μm: Intrinsic evaluation and application to the impurity analysis of griseofulvin. Talanta 122, 122–129 (2014).
- 89 . Quantification of plasma homocitrulline using hydrophilic interaction liquid chromatography (HILIC) coupled to tandem mass spectrometry. Anal. Bioanal. Chem. 402(4), 1635–1641 (2012).
- 90 Measurement of free carnitine and acylcarnitines in plasma by HILIC-ESI-MS/MS without derivatization. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 932, 12–18 (2013).
- 91 Development and validation of a hydrophilic interaction liquid chromatography with tandem mass spectrometry method for the simultaneous detection and quantification of etilefrine and oxilofrine in equine blood plasma and urine. J. Sep. Sci. 37(21), 3015–3023 (2014).
- 92 . Nano LC: principles, evolution, and state-of-the-art of the technique. LCGC North America 29(10), 926e34 (2011).
- 93 . Miniaturized separation techniques in glycomic investigations. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 841(1–2), 65–78 (2006).
- 94 . Dealing with real samples: sample pre-treatment in microfluidic systems. Lab Chip 3(1), 11–19 (2003).
- 95 . Miniaturization and parallelization of biological and chemical assays in microfluidic devices. Chem. Biol. 17(10), 1052–1065 (2010).
- 96 . Biological sample preparation: attempts on productivity increasing in bioanalysis. Bioanalysis 6(12), 1691–1710 (2014).
- 97 . Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode. Anal. Chim. Acta 685(1), 103–110 (2011).
- 98 . Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide. Anal. Chem. 77(21), 6954–6959 (2005).
- 99 Zwitterionic-type hydrophilic interaction nano-liquid chromatography of complex and high mannose glycans coupled with electrospray ionisation high resolution time of flight mass spectrometry. J. Chromatogr. A. 1218(37), 6419–6425 (2011).
- 100 . Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics. Electrophoresis 32(24), 3456–3466 (2011).
- 101 . Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry. Mass Spectrom. Rev. 28, 192–206 (2009).
- 102 . Differential chemical derivatization integrated with chromatographic separation for analysis of isomeric sialylated N-glycans: a nano-hydrophilic interaction liquid chromatography-MS platform. Anal. Chem. 85(17), 8421–8428 (2013).
- 103 . Hydrophilic interaction 10 μm I.D. porous layer open tubular columns for ultratrace glycan analysis by liquid chromatography-mass spectrometry. J. Chromatogr. A. 1216(8), 1223–1231 (2009).
- 104 . Miniaturization through lab-on-a-chip: utopia or reality for routine laboratories? A review. Anal. Chim. Acta 740, 1–11 (2012).
- 105 Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis. J. Chromatogr. A. 1218(23), 3669–3674 (2011).
- 106 . Performance characteristics of a new hybrid triple quadrupole time-of-flight tandem mass spectrometry. Anal. Chem. 83(13), 5442–5446 (2011).
- 107 A chip-based amide-HILIC LC/MS platform for glycosaminoglycan glycomics profiling. Proteomics 9(3), 686–695 (2009).
- 108 Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow. Anal. Chem. 82(2), 516–522 (2010).
- 109 Improved liquid chromatography-MS/MS of heparan sulfate oligosaccharides via chip-based pulsed makeup flow. Anal. Chem. 83(21), 8222–8229 (2011).
- 110 . The fundamental aspects and applications of Agilent HPLC-Chip. J. Sep. Sci. 30(10), 1427–1434 (2007).
- 111 . Lipidomic profiling of biological tissues using off-line two-dimensional high-performance liquid chromatography-mass spectrometry. J. Chromatogr. A. 1218(31), 5146–5156 (2011).
- 112 . A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis. J. Chromatogr. A. 1321, 65–72 (2013).
- 113 Two-dimensional LC–MS/MS to enhance ceramide and phosphatidylcholine species profiling in mouse liver. Biomed. Chromatogr. 28(9), 1284–1293 (2014).
- 114 Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectrometry. Anal. Biochem. 417(1), 80–88 (2011).
- 115 . Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC and ZIC-cHILIC) provide high resolution separation and increase sensitivity in proteome analysis. Anal. Chem. 83(9), 3440–3447 (2011).
- 116 . ZIC-cHILIC as a fractionation method for sensitive and powerful shotgun proteomics. Nat. Protoc. 7(11), 2041–2055 (2012).
- 117 . Investigation of an on-line two-dimensional chromatographic approach for peptide analysis in plasma by LC–MS-MS. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 877(20–21), 1873–1881 (2009).
- 118 Fully automatable two-dimensional hydrophilic interaction liquid chromatography-reversed phase liquid chromatography with online tandem mass spectrometry for shotgun proteomics. J. Sep. Sci. 35(14), 1755–1763 (2012).
- 119 . Combining hydrophilic interaction chromatography (HILIC) and isotope tagging for off-Line LC-NMR applications in metabolite analysis. Metabolites 3(3), 575–591 (2013).
- 120 . Two-dimensional liquid chromatography/mass spectrometry/mass spectrometry separation of water-soluble metabolites. J. Chromatogr. A. 1217(52), 8161–8166 (2010).
- 121 . Design and development of a two-dimensional system based on hydrophilic and reversed-phase liquid chromatography with on-line sample treatment for the simultaneous separation of excreted xenobiotics and endogenous metabolites in urine. Biomed. Chromatogr. 29(8), 1190–1196 (2015).
- 122 . Towards a generic variable column length method development strategy for samples with a large variety in polarity. J. Chromatogr. A. 1372, 174–186 (2014).
- 123 . Hydrophilic interaction liquid chromatography (HILIC) in proteomics. Anal. Bioanal. Chem. 391(1), 151–159 (2008).
- 124 . Nontargeted lipidomic characterization of porcine organs using hydrophilic interaction liquid chromatography and off-line two-dimensional liquid chromatography-electrospray ionization mass spectrometry. Lipids 48(9), 915–928 (2013).
- 125 . Development and optimization of on-line 2-dimensional chromatographic approaches for eliminating matrix effects and improving bioanalysis of peptides in human plasma using UHPLC–MS/MS. Drug Test. Anal. 6(5), 415–425 (2014).
- 126 . Hydrophilic interaction liquid chromatography as second dimension in multidimensional chromatography with an anionic trapping strategy: application to prostate-specific antigen quantification. J. Chromatogr. A. 1354, 75–84 (2014).
- 127 . On-line desorption of dried blood spots coupled to hydrophilic interaction/reversed-phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites. J. Sep. Sci. 33(6–7), 873–879 (2010).
- 128 . On-line comprehensive two-dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part I: orthogonality and practical peak capacity considerations. J. Chromatogr. A. 1262, 148–159 (2012).
- 129 Selection of column dimensions and gradient conditions to maximize the peak-production rate in comprehensive off-line two-dimensional liquid chromatography using monolithic columns. Anal. Chem. 82(16), 7015–7020 (2010).
- 130 . Stop-flow comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection for phospholipid analysis. J. Chromatogr. A. 1278, 46–53 (2013).
- 131 . Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 796(2), 209–224 (2003).
- 132 . Bioanalytical hydrophilic interaction chromatography: recent challenges, solutions and applications. Bioanalysis 1(1), 239–253 (2009).
- 133 . Determination of phenylephrine in human plasma using ultra-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 915–916, 28–32 (2013).
- 134 . UPLC–MS/MS measurement of S-nitrosoglutathione (GSNO) in human plasma solves the S-nitrosothiol concentration enigma. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 927, 147–157 (2013).
- 135 . Rapid and sensitive HILIC-MS/MS analysis of carnitine and acetylcarnitine in biological fluids. Anal. Bioanal. Chem. 405(23), 7397–7404 (2013).
- 136 . Comparison of reversed-phase and hydrophilic interaction liquid chromatography for the quantification of ephedrines using medium-resolution accurate mass spectrometry. J. Chromatogr. A. 1289, 37–46 (2013).
- 137 . Hydrophilic interaction liquid chromatography and accurate mass measurement for quantification and confirmation of morphine, codeine and their glucuronide conjugates in human urine. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 878(29), 2959–2966 (2010).
- 138 Hydrophilic interaction chromatography using a metre-scale monolithic silica capillary column for proteomics LC–MS. Anal. Chem. 86, 3817–3824 (2014).
- 139 Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry with single internal standard and response factor approach. Anal Chem. 84(22), 10064–10070 (2012).
- 140 . Phospholipidomics of human blood microparticles. Anal. Chem. 85(13), 6405–6413 (2013).
- 141 Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis. Anal. Chim. Acta 866, 59–68 (2015).
- 142 Quantitative hydrophilic interaction chromatography–mass spectrometry analysis of N-acetylneuraminic acid and N-acetylmannosamine in human plasma. J. Chromatogr. B. 1000, 105–111 (2015).
- 143 . Hydrophilic Interacion Liquid Chromatography (HILIC) and Advanced Applications. CRC Press, Boca Raton, FL, USA, 477–485 (2011).
- 144 . NP/HILIC-ELSD separation of phospholipid classes and application to preliminary analysis of plasma low density lipoproteins. J. Liq. Chromatogr. Relat. Technol. 38(2), 243–250 (2014).
- 145 . Comparison of the sensitivity of evaporative universal detectors and LC/MS in the HILIC and the reversed-phase HPLC modes. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 877(32), 4133–4139 (2009).
- 146 . Comparison of two aerosol-based detectors for the analysis of gabapentin in pharmaceutical formulations by hydrophilic interaction chromatography. Talanta 85(5), 2301–2306 (2011).
- 147 . Charged aerosol detection in pharmaceutical analysis. J. Pharm. Biomed. Anal. 69, 50–63 (2012).
- 148 . Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection. J. Chromatogr. A. 1218(25), 3854–3861 (2011).
- 149 . Importance of matrix effects in LC–MS/MS bioanalysis. Bioanalysis. 5(19), 2331–2332 (2013).
- 150 . Potential of HILIC-MS in quantitative bioanalysis of drugs and drug metabolites. J. Sep. Sci. 31(9), 1481–1491 (2008).
- 151 . Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC–MS/MS. Anal. Chem. 75(13), 3019–3030 (2003).
- 152 The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compounds. Rapid Commun. Mass Spectrom. 13(12), 1175–1185 (1999).
- 153 Quantitative screening and matrix effect studies of drug discovery compounds in monkey plasma using fastgradient liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 15(24), 2481–2487 (2001).
- 154 . Tracing and separating plasma components causing matrix effects in hydrophilic interaction chromatography-electrospray ionization mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 923–924, 83–91 (2013).
- 155 . Selection of HILIC columns to handle matrix effect due to phospholipids. Bioanalysis 1(1), 57–62 (2009).
- 156 . Utilization of hydrophilic-interaction LC to minimize matrix effects caused by phospholipids. Bioanalysis 4(16), 2049–2058 (2012).
- 157 . Determination of hyaluronic acid in a chitosan-based formulation by RP C18 and HILIC LC – ESI-MS: an evaluation of matrix effect. Chromatographia 76(23–24), 1761–1766 (2013).