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Research Article

Kaempferol attenuates the effects of XIST/miR-130a/STAT3 on inflammation and extracellular matrix degradation in osteoarthritis

    Yaosheng Xiao‡

    Medical College of Soochow University, Suzhou, 320505, China

    Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China

    Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, 320505, China

    ‡Authors contributed equally

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    ,
    Lulin Liu‡

    Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China

    ‡Authors contributed equally

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    ,
    Yizhou Zheng

    College of Pharmacy, Gannan Medical University, Ganzhou, 341000, China

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    ,
    Wuyang Liu

    Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China

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    &
    Youjia Xu

    *Author for correspondence:

    E-mail Address: xuyoujia@suda.edu.cn

    Medical College of Soochow University, Suzhou, 320505, China

    Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, 320505, China

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    Published Online:14 Jun 2021https://doi.org/10.4155/fmc-2021-0127

    Abstract

    Aim: To investigate whether kaempferol exhibited protective effects on osteoarthritis chondrocytes by modulating the XIST/miR-130a/STAT3 axis. Methods: qRT-PCR and western blot assays were used for gene and protein determination. Dual luciferase reporter and RNA immunoprecipitation assays were employed to study the interaction between miRNA and lncRNA or genes. Results: Kaempferol decreased proinflammatory cytokine production and extracellular matrix degradation in C28/I2 cells. Additionally, kaempferol ameliorated XIST expression and enhanced miR-130a expression. XIST interacted with miR-130a, and STAT3 was identified as a target of miR-130a. Knockdown of XIST expression suppressed proinflammatory cytokine production and extracellular matrix degradation in C28/I2 cells. Overexpression of STAT3 rescued the effects of XIST knockdown. Conclusion: Kaempferol inhibited inflammation and extracellular matrix degradation by modulating the XIST/miR-130a/STAT3 axis in chondrocytes.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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