Review

Analysis of bacterial biofilms using NMR-based metabolomics

Bo Zhang

Department of Chemistry, University of Nebraska-Lincoln, 722 Hamilton Hall, Lincoln, NE 68588–0304, USA

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Robert Powers

* Author for correspondence

Department of Chemistry, University of Nebraska-Lincoln, 722 Hamilton Hall, Lincoln, NE 68588–0304, USA.

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Email the corresponding author at rpowers3@unl.edu

Published Online:16 Jul 2012https://doi.org/10.4155/fmc.12.59

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Abstract

Infectious diseases can be difficult to cure, especially if the pathogen forms a biofilm. After decades of extensive research into the morphology, physiology and genomics of biofilm formation, attention has recently been directed toward the analysis of the cellular metabolome in order to understand the transformation of a planktonic cell to a biofilm. Metabolomics can play an invaluable role in enhancing our understanding of the underlying biological processes related to the structure, formation and antibiotic resistance of biofilms. A systematic view of metabolic pathways or processes responsible for regulating this ‘social structure’ of microorganisms may provide critical insights into biofilm-related drug resistance and lead to novel treatments. This review will discuss the development of NMR-based metabolomics as a technology to study medically relevant biofilms. Recent advancements from case studies reviewed in this manuscript have shown the potential of metabolomics to shed light on numerous biological problems related to biofilms.

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Published online 16 July 2012

Published in print June 2012

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Financial & competing interests disclosure

This manuscript was supported in part by funds from the NIH (AI087668), and from the NIH National Center for Research Resources (P20 RR-17675). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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