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Analysis of insoluble proteins

Sarah Trimpin

Bill Brizzard

Sarah Trimpin

Department of Chemistry, Wayne State University, Department of Chemistry, Detroit, Michigan, USA

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Bill Brizzard

^*Address correspondence to Bill Brizzard, Indiana University Research and Technology Corporation, 501 North Morton Street, Suite 204, Bloomington, IN, 47401, USA. email:

E-mail Address: bbrizzar@iu.edu

Indiana University Research and Technology Corporation, Bloomington, Indiana, USA

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Published Online:25 Apr 2018https://doi.org/10.2144/000113168

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Abstract

The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20–30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time-consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.

Keywords:

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