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Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking

Isabelle Migneault

Catherine Dartiguenave

Michel J. Bertrand

Karen C. Waldron

Isabelle Migneault

Université de Montréal, Montréal, Canada

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Catherine Dartiguenave

Université de Montréal, Montréal, Canada

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Michel J. Bertrand

Université de Montréal, Montréal, Canada

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Karen C. Waldron

^*Address correspondence to: Karen C. Waldron, Department of Chemistry, Université de Montréal, C.P. 6128, Succ. Centre-ville, Montréal, Canada, H3C 3J7. e-mail:

E-mail Address: karen.waldron@umontreal.ca

Université de Montréal, Montréal, Canada

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Published Online:6 Jun 2018https://doi.org/10.2144/04375RV01

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Abstract

Glutaraldehyde possesses unique characteristics that render it one of the most effective protein crosslinking reagents. It can be present in at least 13 different forms depending on solution conditions such as pH, concentration, temperature, etc. Substantial literature is found concerning the use of glutaraldehyde for protein immobilization, yet there is no agreement about the main reactive species that participates in the crosslinking process because monomeric and polymeric forms are in equilibrium. Glutaraldehyde may react with proteins by several means such as aldol condensation or Michael-type addition, and we show here 8 different reactions for various aqueous forms of this reagent. As a result of these discrepancies and the unique characteristics of each enzyme, crosslinking procedures using glutaraldehyde are largely developed through empirical observation. The choice of the enzyme-glutaraldehyde ratio, as well as their final concentration, is critical because insolubilization of the enzyme must result in minimal distortion of its structure in order to retain catalytic activity. The purpose of this paper is to give an overview of glutaraldehyde as a crosslinking reagent by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.

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- Enzymatic reduction of CO2 to formic acid using FDH immobilized on natural zeolite
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- Cryofixation of Inactivated Hantavirus-Infected Cells as a Method for Obtaining High-Quality Ultrastructural Preservation for Electron Microscopic Studies
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- The choice of biopolymer is crucial to trigger angiogenesis with vascular endothelial growth factor releasing coatings
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- An efficient decolorization of methyl orange dye by laccase from Marasmiellus palmivorus immobilized on chitosan-coated magnetic particles
  Biocatalysis and Agricultural Biotechnology, Vol. 30
- Immobilisation of cellulase on vermiculite and the effects on enzymatic kinetics and thermodynamics
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- Improved immobilization of lipase from Thermomyces lanuginosus on a new chitosan-based heterofunctional support: Mixed ion exchange plus hydrophobic interactions
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- Dual immobilization of α-amylase and horseradish peroxidase via electrospinning: A proof of concept study
  International Journal of Biological Macromolecules, Vol. 163
- A Comprehensive Review of the Covalent Immobilization of Biomolecules onto Electrospun Nanofibers
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- Stabilization of immobilized l ‐arabinose isomerase for the production of d ‐tagatose from d ‐galactose
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- Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species
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- In vivo evaluation of Vivere bovine pericardium valvular bioprosthesis with a new anti‐calcifying treatment
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- Optimization of immobilization conditions of Bacillus atrophaeus FSHM2 lipase on maleic copolymer coated amine-modified graphene oxide nanosheets and its application for valeric acid esterification
  International Journal of Biological Macromolecules, Vol. 162
- Biocide Chemistries Used in Nitrite Inhibited Closed Loop Cooling Medium at High pH
  27 October 2020
- Functionalization of Electrospun Nanofibers and Fiber Alignment Enhance Neural Stem Cell Proliferation and Neuronal Differentiation
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- Design of an Automated Electrochemical Biosensor Modified with Phenylboronic Acid to Study Glycoprotein Immobilization
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- Three dimensional spongy fibroin scaffolds containing keratin/vanillin particles as an antibacterial skin tissue engineering scaffold
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- Structure and properties of lipase activated by cellulose-silica polyethersulfone membrane for production of pentyl valerate
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  Colloids and Surfaces B: Biointerfaces, Vol. 194
- Current methods of collagen cross-linking: Review
  International Journal of Biological Macromolecules, Vol. 161
- Strategies, challenges and opportunities of enzyme immobilization on porous silicon for biosensing applications
  Journal of Environmental Chemical Engineering, Vol. 8, No. 5
- Modifying alcalase activity and stability by immobilization onto chitosan aiming at the production of bioactive peptides by hydrolysis of tilapia skin gelatin
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- Fixed versus live endothelial cells: The effect of glutaraldehyde fixation manifested by characteristic bands on the Raman spectra of cells
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- Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
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- Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation
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- Corrosive Esophagitis and Gastritis Induced by Glutaraldehyde Ingestion
  The Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol. 20, No. 3
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- Entrapment of glutaraldehyde‐crosslinked cells from Aspergillus oryzaeIPT ‐301 in calcium alginate for high transfructosylation activity
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- Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant
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- Polymer Discs with High Interfacial Adhesion Fabricated from Hot-Pressing of Microspheres
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- Entrapment of enzyme aggregates in chitosan beads for aroma release in white wines
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  Reactive and Functional Polymers, Vol. 152
- Cytotoxicity and UV Light Absorption in Biopolymeric Membranes from Native Vegetation of Mexico
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- Synchrotron FTIR Light Reveals Signal Changes of Biofunctionalized Magnetic Nanoparticle Attachment on Salmonella sp.
  Journal of Nanomaterials, Vol. 2020
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- Interaction of the chaperones alpha B-crystallin and CHIP with fibrillar alpha-synuclein: Effects on internalization by cells and identification of interacting interfaces
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- Poly(vinyl) alcohol crosslinked composite packaging film containing gold nanoparticles on shelf life extension of banana
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- Rapidly and Precisely Cross-Linked Enzymes Using Bio-Orthogonal Chemistry from Cell Lysate for the Synthesis of ( S )-1-(2,6-Dichloro-3-fluorophenyl) Ethanol
  7 April 2020 | ACS Sustainable Chemistry & Engineering, Vol. 8, No. 16
- Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration
  17 April 2020 | Frontiers in Bioengineering and Biotechnology, Vol. 8
- IMMOBILIZATION OF Bacillus subtilis E6-5 PROTEASE AND COMMERCIAL PROTEASE IN NANOFIBRILS CONTAINING DIFFERENT AMINO ACIDS
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- Functionalized κ-carrageenan/hyperbranched poly(amidoamine)for protease immobilization: Thermodynamics and stability studies
  International Journal of Biological Macromolecules, Vol. 148
- Assessment of the Effect of Secondary Fixation on the Structure of Meat Products Prepared for Scanning Electron Microscopy
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- Antigen Retrieval for Light and Electron Microscopy
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- Multimodal detection and analysis of a new type of advanced Heinz body-like aggregate (AHBA) and cytoskeleton deformation in human RBCs
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- Flexible platinum electrodes as electrochemical sensor and immunosensor for Parkinson's disease biomarkers
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- Glutaraldehyde Cross-Linking of Oligolysines Coating DNA Origami Greatly Reduces Susceptibility to Nuclease Degradation
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  3 July 2020
- Achieving sterility in biomedical and pharmaceutical products (part-I): thermal, chemical, and filtration sterilization
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- New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part A: Optimization of a crosslinked film support based on chitosan and cellulose nanocrystals (CNC)
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- Further stabilization of lipase from Pseudomonas fluorescens immobilized on octyl coated nanoparticles via chemical modification with bifunctional agents
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- Unconventional Oil and Gas Energy Systems: An Unidentified Hotspot of Antimicrobial Resistance?
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- A disposable acetylcholine esterase sensor for As( iii ) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis
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- AgarFix: Simple and accessible stabilization of challenging single-particle cryo-EM specimens through crosslinking in a matrix of agar
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- An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam
  International Journal of Biological Macromolecules, Vol. 125
- The influences of 3,4-dihydroxybenzaldehyde on the microstructure and stability of collagen fibrils
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- Treatment of Surface Plasmon Resonance (SPR) Background in Total Internal Reflection Ellipsometry: Characterization of RNA Polymerase II Film Formation
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  1 March 2019 | Brazilian Journal of Chemical Engineering, Vol. 36, No. 1
- Cooperativity of covalent attachment and ion exchange on alcalase immobilization using glutaraldehyde chemistry: Enzyme stabilization and improved proteolytic activity
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- Self-Assembly of siRNA/PEG- b -Catiomer at Integer Molar Ratio into 100 nm-Sized Vesicular Polyion Complexes (siRNAsomes) for RNAi and Codelivery of Cargo Macromolecules
  7 February 2019 | Journal of the American Chemical Society, Vol. 141, No. 8
- Effect of the Cross-Linking Modification on Corn Stalk Fiber Reinforced Polypropylene Composites
  18 February 2019 | IOP Conference Series: Materials Science and Engineering, Vol. 472
- Assembly of Protein–Polysaccharide Complexes for Delivery of Bioactive Ingredients: A Perspective Paper
  14 January 2019 | Journal of Agricultural and Food Chemistry, Vol. 67, No. 5
- Efficient immobilization of firefly luciferase in a metal organic framework: Fe-MIL-88(NH2) as a mighty support for this purpose
  Enzyme and Microbial Technology, Vol. 121
- Catalytic phenol removal using entrapped cross-linked laccase aggregates
  International Journal of Biological Macromolecules, Vol. 122
- Preparation of Crosslinked Enzyme Aggregates of a Thermostable Cyclodextrin Glucosyltransferase from Thermoanaerobacter sp. Critical Effect of the Crosslinking Agent
  30 January 2019 | Catalysts, Vol. 9, No. 2
- “Fix and assay”: separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative
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- Cross-linked Enzyme Aggregates: Current Developments and Applications
  4 September 2019
- Protein-Based Hydrogels
  5 January 2019
- Nanostructures of gluten for encapsulation of food ingredients
- Immobilized Enzymes and Their Applications
- Enzymes as Analytical Tools for the Assessment of Food Quality and Food Safety
- In vivo self-assembly of small diameter pulmonary visceral pleura artery graft
  Acta Biomaterialia, Vol. 83
- Magnetic cross-linked enzyme aggregates of acrylamidase from Cupriavidus oxalaticus ICTDB921 for biodegradation of acrylamide from industrial waste water
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- Optimization of a dual-functional biocatalytic system for continuous hydrolysis of lactose in milk
  Journal of Bioscience and Bioengineering, Vol. 127, No. 1
- Polypyrrole/polyethylenimine Hybrid Film with Improved Potentiometric Response for Urea Sensing Application
  Materials Today: Proceedings, Vol. 17
- Protection of tissue physicochemical properties using polyfunctional crosslinkers
  17 December 2018 | Nature Biotechnology, Vol. 37, No. 1
- Desolvation Conditions for Production of Sulfasalazine Based Albumin Nanoparticles: Physical Properties
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- Immobilized Enzymes in Biosensor Applications
  2 January 2019 | Materials, Vol. 12, No. 1
- Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development
  18 January 2019 | Sensors, Vol. 19, No. 2
- 1T-Phase Tungsten Chalcogenides (WS 2 , WSe 2 , WTe 2 ) Decorated with TiO 2 Nanoplatelets with Enhanced Electron Transfer Activity for Biosensing Applications
  10 December 2018 | ACS Applied Nano Materials, Vol. 1, No. 12
- pH Variation as a Simple and Selective Pathway for Obtaining Nanoparticle or Nanocapsule Polysaccharides
  3 December 2018 | Langmuir, Vol. 34, No. 51
- Piperine, an alkaloid inhibiting the super-relaxed state of myosin, binds to the myosin regulatory light chain
  Archives of Biochemistry and Biophysics, Vol. 659
- Chitosan nanoparticles via high-pressure homogenization-assisted miniemulsion crosslinking for mixed-matrix membrane adsorbers
  Carbohydrate Polymers, Vol. 201
- Saponin loaded montmorillonite-human serum albumin nanocomposites as drug delivery system in colorectal cancer therapy
  Applied Clay Science, Vol. 166
- Design and characterization of immobilized biocatalyst with lipase activity onto magnetic magnesium spinel nanoparticles: A novel platform for biocatalysis
  Colloids and Surfaces B: Biointerfaces, Vol. 172
- Oriented covalent immobilization of recombinant protein A on the glutaraldehyde activated agarose support
  International Journal of Biological Macromolecules, Vol. 120
- In Vitro transdermal delivery of sesamol using oleic acid chemically-modified gelatin nanoparticles as a potential breast cancer medication
  Journal of Drug Delivery Science and Technology, Vol. 48
- Development of microreactors with surface-immobilized biocatalysts for continuous transamination
  New Biotechnology, Vol. 47
- Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella
  Sensors and Actuators B: Chemical, Vol. 276
- Preliminary study of a non-invasive portable device for continuous monitoring of blood alcohol concentration
- Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
  24 November 2018 | Plant Methods, Vol. 14, No. 1
- α-Chymotrypsin Immobilized on a Low-Density Polyethylene Surface Successfully Weakens Escherichia coli Biofilm Formation
  12 December 2018 | International Journal of Molecular Sciences, Vol. 19, No. 12
- Computer‐aided identification of a novel pyruvate kinase M2 activator compound
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- An environmentally-friendly chitosan-lysozyme biocomposite for the effective removal of dyes and heavy metals from aqueous solutions
  Carbohydrate Polymers, Vol. 199
- The development of nanobiocatalysis via the immobilization of cellulase on composite magnetic nanomaterial for enhanced loading capacity and catalytic activity
  International Journal of Biological Macromolecules, Vol. 119
- Kinetic and thermodynamic features of nanomagnetic cross-linked enzyme aggregates of naringinase nanobiocatalyst in naringin hydrolysis
  International Journal of Biological Macromolecules, Vol. 119
- Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity Problems
  26 October 2018 | Catalysts, Vol. 8, No. 11
- Direct Evaluation of Live Uropathogenic Escherichia coli Adhesion and Efficiency of Antiadhesive Compounds Using a Simple Microarray Approach
  4 October 2018 | Analytical Chemistry, Vol. 90, No. 20
- Protease-based cross-linked enzyme aggregates with improved catalytic stability, silver removal, and dehairing potentials
  International Journal of Biological Macromolecules, Vol. 118
- Encapsulation and immobilization of ficin extract in electrospun polymeric nanofibers
  International Journal of Biological Macromolecules, Vol. 118
- Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study
  Journal of the Taiwan Institute of Chemical Engineers, Vol. 91
- Combined Cross-Linked Enzyme Aggregates as Biocatalysts
  17 October 2018 | Catalysts, Vol. 8, No. 10
- Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors
  19 October 2018 | Catalysts, Vol. 8, No. 10
- Chemical and Enzymatic Protein Cross-Linking To Improve Flocculant Properties
  28 August 2018 | ACS Sustainable Chemistry & Engineering, Vol. 6, No. 10
- Enhanced reusability and activity: DNA directed immobilization of enzyme on polydopamine modified magnetic nanoparticles
  Biochemical Engineering Journal, Vol. 137
- Immobilization and stabilization of alcohol dehydrogenase on polyvinyl alcohol fibre
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- Use of chitosan heterofunctionality for enzyme immobilization: β-galactosidase immobilization for galacto-oligosaccharide synthesis
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- Crosslinked enzyme aggregates (CLEA) of phytase with soymilk proteins
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- Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures
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- Influence of Glutaraldehyde Cross-Linking Modes on the Recyclability of Immobilized Lipase B from Candida antarctica for Transesterification of Soy Bean Oil
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- IMOBILIZAÇÃO DA BIOMASSA CATALÍTICA DE Aspergillus oryzae IPT-301 VISANDO A PRODUÇÃO DE FRUTOOLIGOSSACARÍDEOS
  1 September 2018
- Microdevice‐based solid‐phase polymerase chain reaction for rapid detection of pathogenic microorganisms
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- Immobilization of Carboxypeptidase A into Modified Chitosan Matrixes by Covalent Attachment
  6 February 2018 | Applied Biochemistry and Biotechnology, Vol. 185, No. 4
- An amperometric glutamate biosensor for monitoring glutamate release from brain nerve terminals and in blood plasma
  Analytica Chimica Acta, Vol. 1022
- Preparation of a novel nanobiocatalyst by immobilizing penicillin acylase onto magnetic nanocrystalline cellulose and its use for efficient synthesis of cefaclor
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- Full length histone H3 conjugated electrochemical biosensor for extracellular proteolytic Cathepsin L activity detection
  Sensors and Actuators B: Chemical, Vol. 267
- Sensitive Leptospira DNA detection using tapered optical fiber sensor
  24 April 2018 | Journal of Biophotonics, Vol. 11, No. 8
- Enzyme Immobilization on Inorganic Surfaces for Membrane Reactor Applications: Mass Transfer Challenges, Enzyme Leakage and Reuse of Materials
  5 June 2018 | Advanced Synthesis & Catalysis, Vol. 360, No. 14
- Kinetic and thermodynamic studies on the enzymatic synthesis of wax ester catalyzed by lipase immobilized on glutaraldehyde-activated rice husk particles
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- Glutaraldehyde as Ambient Temperature Crosslinking Agent of Latex Films from Natural Rubber Grafted with Poly(diacetone acrylamide)
  5 February 2018 | Journal of Polymers and the Environment, Vol. 26, No. 7
- Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads
  Food Chemistry, Vol. 253
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  International Journal of Biological Macromolecules, Vol. 114
- Photolysis of glutaraldehyde in brine: A showcase study for removal of a common biocide in oil and gas produced water
  Journal of Hazardous Materials, Vol. 353
- Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils
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- 1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates
  7 May 2018 | Biotechnology Progress, Vol. 34, No. 4
- An Electrochemical Immunosensor Based on Poly(Thionine)-Modified Carbon Paste Electrode for the Determination of Prostate Specific Antigen
  IEEE Sensors Journal, Vol. 18, No. 12
- Mechanical and spectroscopic characterization of crosslinked zein films cast from solutions of acetic acid leading to a new mechanism for the crosslinking of oleic acid plasticized zein films
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  Process Biochemistry, Vol. 69
- Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins
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- Highly porous core–shell chitosan beads with superb immobilization efficiency for Lactobacillus reuteri 121 inulosucrase and production of inulin-type fructooligosaccharides
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- Bone toughness at the molecular scale: A model for fracture toughness using crosslinked osteopontin on synthetic and biogenic mineral substrates
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- In vitro nuclear receptor inhibition and cytotoxicity of hydraulic fracturing chemicals and their binary mixtures
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- Designing cross-linked xylanase aggregates for bioconversion of agroindustrial waste biomass towards potential production of nutraceuticals
  International Journal of Biological Macromolecules, Vol. 111
- The performance of microbial lipase immobilized onto polyolefin supports for hydrolysis of high oleate sunflower oil
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- Mg2+ binding triggers rearrangement of the IM30 ring structure, resulting in augmented exposure of hydrophobic surfaces competent for membrane binding
  Journal of Biological Chemistry, Vol. 293, No. 21
- Preparation and Evaluation of Coal Fly Ash/Chitosan Composites as Magnetic Supports for Highly Efficient Cellulase Immobilization and Cellulose Bioconversion
  14 May 2018 | Polymers, Vol. 10, No. 5
- Enhanced Selectivity by Passivation: Molecular Imprints for Viruses with Exceptional Binding Properties
  5 March 2018 | Analytical Chemistry, Vol. 90, No. 9
- Binding Specificities of Nanobody•Membrane Protein Complexes Obtained from Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry
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- Effect of the Presence of Surfactants and Immobilization Conditions on Catalysts’ Properties of Rhizomucor miehei Lipase onto Chitosan
  10 October 2017 | Applied Biochemistry and Biotechnology, Vol. 184, No. 4
- Processed gellan gum beads as covalent immobilization carriers
  Biocatalysis and Agricultural Biotechnology, Vol. 14
- Whey protein isolate for the preparation of covalent immobilization beads
  Biocatalysis and Agricultural Biotechnology, Vol. 14
- Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates
  21 April 2018 | Catalysts, Vol. 8, No. 4
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  19 January 2018 | ELECTROPHORESIS, Vol. 39, No. 7
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  30 January 2018 | ACS Biomaterials Science & Engineering, Vol. 4, No. 3
- A novel ultra‐low energy reverse osmosis membrane modified by chitosan with glutaraldehyde crosslinking
  10 November 2017 | Journal of Applied Polymer Science, Vol. 135, No. 10
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- Efficient gene delivery by oligochitosan conjugated serum albumin: Facile synthesis, polyplex stability, and transfection
  Carbohydrate Polymers, Vol. 183
- Enhancement of n -3 polyunsaturated fatty acid glycerides in Sardine oil by a bioimprinted cross-linked Candida rugosa lipase
  Enzyme and Microbial Technology, Vol. 110
- Immobilization of β-1,3-1,4-glucanase from Bacillus sp. on porous silica for production of β-glucooligosaccharides
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- Stabilization of dimeric β-glucosidase from Aspergillu s nige r via glutaraldehyde immobilization under different conditions
  Enzyme and Microbial Technology, Vol. 110
- Structure and properties of hybrid biopolymer particles fabricated by co-precipitation cross-linking dissolution procedure
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- Effect of micellar κ-casein dissociation on the formation of soluble protein complexes and acid gel properties
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- Injectable Hydrogels for Localized Chemotherapy and Radiotherapy in Brain Tumors
  Journal of Pharmaceutical Sciences, Vol. 107, No. 3
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- Immobilization of CALB on lysine-modified magnetic nanoparticles: influence of the immobilization protocol
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- Optimization of Enzyme Co-Immobilization with Sodium Alginate and Glutaraldehyde-Activated Chitosan Beads
  31 July 2017 | Applied Biochemistry and Biotechnology, Vol. 184, No. 2
- Fabrication and characterization of a novel collagen-catechol hydrogel
  13 December 2017 | Journal of Biomaterials Applications, Vol. 32, No. 7
- A Phenotyping Method of Giant Cells from Root-Knot Nematode Feeding Sites by Confocal Microscopy Highlights a Role for CHITINASE-LIKE 1 in Arabidopsis
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- Role of Biocatalysis in Sustainable Chemistry
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- Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy
  16 November 2017 | The EMBO Journal, Vol. 37, No. 1
- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques
  19 June 2018
- Protein-Based Hydrogels
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- Carrier-Free Enzyme Immobilization by Cross-Linked Enzyme Aggregates (CLEA) Technology
  24 January 2019
- Synthesis and Properties Modified Feather Keratin-Based Motor Oil Sorbing Cryogels with High Oil Holding Capacity
  30 December 2016 | Journal of Polymers and the Environment, Vol. 26, No. 1
- More Chemistry
- Coacervation Technique as an Encapsulation and Delivery Tool for Hydrophobic Biofunctional Compounds
- Natural and semisynthetic polymers in pharmaceutical nanotechnology
- Conversion of levulinic acid into alkyl levulinates: Using lipase immobilized on meso-molding three-dimensional macroporous organosilica as catalyst
  Bioresource Technology, Vol. 247
- A systematic approach of chitosan nanoparticle preparation via emulsion crosslinking as potential adsorbent in wastewater treatment
  Carbohydrate Polymers, Vol. 180
- The effect of NDGA-modified etchant on the enzymatic degradation resistance and mechanical properties of collagen matrix
  Chinese Chemical Letters, Vol. 29, No. 1
- Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag™ fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol
  1 January 2018 | Green Chemistry, Vol. 20, No. 2
- Engineering magnetically responsive tropoelastin spongy-like hydrogels for soft tissue regeneration
  1 January 2018 | Journal of Materials Chemistry B, Vol. 6, No. 7
- Development of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensor
  1 January 2018 | The Analyst, Vol. 143, No. 16
- Synthesis of luminescent lanthanide complexes within crosslinked protein crystal matrices
  1 January 2018 | CrystEngComm, Vol. 20, No. 16
- Attachment of enzymes to hydrophilic magnetic nanoparticles through DNA-directed immobilization with enhanced stability and catalytic activity
  1 January 2018 | New Journal of Chemistry, Vol. 42, No. 11
- Hexavalent chromium ion and methyl orange dye uptake via a silk protein sericin–chitosan conjugate
  1 January 2018 | RSC Advances, Vol. 8, No. 48
- Encapsulation and dissolution study of bromelain in chitosan-methyl cellulose semi-IPN hydrogel
- Chemical modification of in natura collagen by acidic catalysis – structural characterization and mechanistic features of Cd(II) sorption by solution microcalorimetry
  6 December 2018 | Matéria (Rio de Janeiro), Vol. 23, No. 4
- Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants
  18 January 2018 | Catalysts, Vol. 8, No. 1
- Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes
  23 December 2017 | International Journal of Environmental Research and Public Health, Vol. 15, No. 1
- Advances in Capillary Electrophoretically Mediated Microanalysis for On‐line Enzymatic and Derivatization Reactions
  13 September 2017 | ELECTROPHORESIS, Vol. 39, No. 1
- Lectin corona enhances enzymatic catalysis on the surface of magnetic nanoparticles
  Biochemical Engineering Journal, Vol. 129
- Porous chitosan beads of superior mechanical properties for the covalent immobilization of enzymes
  International Journal of Biological Macromolecules, Vol. 105
- Development of an aptasensor using reduced graphene oxide chitosan complex to detect Salmonella
  Journal of Electroanalytical Chemistry, Vol. 806
- High-performance therapeutic quercetin-doped adhesive for adhesive–dentin interfaces
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- Stabilization of a Lipolytic Enzyme for Commercial Application
  21 March 2017 | Catalysts, Vol. 7, No. 12
- Soy-Based Adhesive Cross-Linked by Phenol-Formaldehyde-Glutaraldehyde
  8 May 2017 | Polymers, Vol. 9, No. 12
- Effect of the Helix–Coil Transition in Bovine Skin Gelatin on Its Associative Phase Separation with Lysozyme
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- Auf Lysin zielende, kovalente Inhibitoren
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- In Situ Healing of Compromised Membranes via Polyethylenimine-Functionalized Silica Microparticles
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- Synthesis of highly swellable hydrogels of water-soluble carboxymethyl chitosan and poly(ethylene glycol)
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- Enzymatic conversion of CO2 to CH3OH via reverse dehydrogenase cascade biocatalysis: Quantitative comparison of efficiencies of immobilized enzyme systems
  Biochemical Engineering Journal, Vol. 127
- Amyloid-like protein nanofibrous membranes as a sensing layer infrastructure for the design of mass-sensitive biosensors
  Biosensors and Bioelectronics, Vol. 97
- Structure and properties of oil palm-based nanocellulose reinforced chitosan nanocomposite for efficient synthesis of butyl butyrate
  Carbohydrate Polymers, Vol. 176
- Chemical fixation to arrest phospholipid signaling for chemical cytometry
  Journal of Chromatography A, Vol. 1523
- Nanoparticulation of bovine serum albumin and poly-d-lysine through complex coacervation and encapsulation of curcumin
  Colloids and Surfaces B: Biointerfaces, Vol. 159
- Preparation and evaluation of BSA-based hydrosol nanoparticles cross-linked with genipin for oral administration of poorly water-soluble curcumin
  International Journal of Biological Macromolecules, Vol. 104
- Improving the integrity of natural biopolymer films used in food packaging by crosslinking approach: A review
  International Journal of Biological Macromolecules, Vol. 104
- Effect of crosslinking on the physical and chemical properties of β-lactoglobulin (Blg) microgels
  Journal of Colloid and Interface Science, Vol. 505
- Modeling the response of exogenously crosslinked tissue to cyclic loading: The effects of permanent set
  Journal of the Mechanical Behavior of Biomedical Materials, Vol. 75
- Immobilization of Lecitase® ultra on recyclable polymer support: application in resolution of trans-methyl (4-methoxyphenyl)glycidate in organic solvents
  Tetrahedron: Asymmetry, Vol. 28, No. 11
- Extracellular low‐n oligomers of tau cause selective synaptotoxicity without affecting cell viability
  18 May 2017 | Alzheimer's & Dementia, Vol. 13, No. 11
- Improvement of covalent immobilization procedure of β-galactosidase from Kluyveromyces lactis for galactooligosaccharides production: Modeling and kinetic study
  18 June 2017 | Biotechnology Progress, Vol. 33, No. 6
- Protein-protein cross-linking and human health: the challenge of elucidating with mass spectrometry
  4 August 2017 | Expert Review of Proteomics, Vol. 14, No. 10
- Improvement in biochemical characteristics of glycosylated phytase through immobilization on nanofibers
  Biocatalysis and Agricultural Biotechnology, Vol. 12
- Chitosan-glutaraldehyde activated calcium pectinate beads as a covalent immobilization support
  Biocatalysis and Agricultural Biotechnology, Vol. 12
- Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate
  Electrochimica Acta, Vol. 251
- Effects of freezing, fixation and dehydration on surface roughness properties of porcine left anterior descending coronary arteries
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- Preparation and characterization of PHMB-based multifunctional microcapsules
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  6 October 2017 | Helvetica Chimica Acta, Vol. 100, No. 10
- Stimuli‐Responsive Dipeptide–Protein Hydrogels through Schiff Base Coassembly
  25 August 2017 | Macromolecular Rapid Communications, Vol. 38, No. 20
- In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization
  10 July 2017 | Macromolecular Materials and Engineering, Vol. 302, No. 10
- Farklı çapraz bağlayıcılar ile hazırlanmış poliakrilamid hidrojellerinin değerlendirilmesi
  29 September 2017 | Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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  21 September 2017 | ChemistrySelect, Vol. 2, No. 27
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  5 September 2017
- Inhibition of Biodegradation of Hydraulic Fracturing Compounds by Glutaraldehyde: Groundwater Column and Microcosm Experiments
  25 August 2017 | Environmental Science & Technology, Vol. 51, No. 17
- Synthesis and Assessment of Novel Gelatin–Chitosan Lactate Cohydrogels for Controlled Delivery and Tissue Engineering Applications
  3 January 2017 | Polymer-Plastics Technology and Engineering, Vol. 56, No. 13
- Immobilization of Recombinant Glucose Isomerase for Efficient Production of High Fructose Corn Syrup
  11 March 2017 | Applied Biochemistry and Biotechnology, Vol. 183, No. 1
- Polyethyleneimine functionalized mesoporous diatomite particles for selective copper recovery from aqueous media
  International Journal of Mineral Processing, Vol. 166
- Preserving enzymatic activity and enhancing biochemical stability of glutathione transferase by soluble additives under free and tethered conditions
  4 May 2017 | Biotechnology and Applied Biochemistry, Vol. 64, No. 5
- PAMAM dendrimer hydrogel film—biocompatible material to an efficient dermal delivery of drugs
  4 August 2017 | Journal of Nanoparticle Research, Vol. 19, No. 8
- Biocatalyst including porous enzyme cluster composite immobilized by two-step crosslinking and its utilization as enzymatic biofuel cell
  Journal of Power Sources, Vol. 360
- Spectroscopic method for the detection of 2,4-dichlorophenoxyacetic acid based on its inhibitory effect towards catalase immobilized on reusable magnetic Fe3O4-chitosan nanocomposite
  Sensors and Actuators B: Chemical, Vol. 247
- Wnt ligand presentation and reception: from the stem cell niche to tissue engineering
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- Preparation of spherical cross-linked lipase aggregates with improved activity, stability and reusability characteristic in water-in-ionic liquid microemulsion
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- Directed immobilization of CGTase: The effect of the enzyme orientation on the enzyme activity and its use in packed-bed reactor for continuous production of cyclodextrins
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- Constructing cellular niche properties by localized presentation of Wnt proteins on synthetic surfaces
  29 June 2017 | Nature Protocols, Vol. 12, No. 7
- Insights into the mechanism of cystatin C oligomer and amyloid formation and its interaction with β-amyloid
  Journal of Biological Chemistry, Vol. 292, No. 27
- New Heterofunctional Supports Based on Glutaraldehyde-Activation: A Tool for Enzyme Immobilization at Neutral pH
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- Optimization of Glutaraldehyde Vapor Treatment for Electrospun Collagen/Silk Tissue Engineering Scaffolds
  2 June 2017 | ACS Omega, Vol. 2, No. 6
- Smart Hydrogels: Application in Bioethanol Production
  14 July 2017
- Formation of Dendrimer Nanoassemblies by Oligomerization-Induced Liquid–Liquid Phase Separation
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- Stability and activity of immobilized trypsin on carboxymethyl chitosan-functionalized magnetic nanoparticles cross-linked with carbodiimide and glutaraldehyde
  Journal of Chromatography B, Vol. 1054
- Immobilization of Cellulase Enzyme on Zinc Ferrite Nanoparticles in Increasing Enzymatic Hydrolysis on Ultrasound-Assisted Alkaline Pretreated Crotalaria Juncea Biomass
  Indian Journal of Science and Technology, Vol. 10, No. 24
- Synthesis and characterization of films based on cross linked blends of poly (vinylalcohol) and poly (vinylpyrrolidone) with glutaraldehyde for tissue engineering application
  19 June 2017 | Materialwissenschaft und Werkstofftechnik, Vol. 48, No. 6
- A Brief Introduction to Traditional Bioconjugate Chemistry
  3 March 2017
- General Dialdehyde Click Chemistry for Amine Bioconjugation
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- Based on DNA Strand Displacement and Functionalized Magnetic Nanoparticles: A Promising Strategy for Enzyme Immobilization
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- Mesoporous phenylalanine ammonia lyase microspheres with improved stability through calcium carbonate templating
  International Journal of Biological Macromolecules, Vol. 98
- Effect of ionic and covalent crosslinking agents on properties of chitosan beads and sorption effectiveness of Reactive Black 5 dye
  Reactive and Functional Polymers, Vol. 114
- Cytotoxicity and molecular effects of biocidal disinfectants (quaternary ammonia, glutaraldehyde, poly(hexamethylene biguanide) hydrochloride PHMB) and their mixtures in vitro and in zebrafish eleuthero-embryos
  Science of The Total Environment, Vol. 586
- A melamine based molecularly imprinted sensor for the determination of 8-hydroxydeoxyguanosine in human urine
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- Stabilized HIV-1 envelope glycoprotein trimers for vaccine use
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- Conductive hydrogel composed of 1,3,5-benzenetricarboxylic acid and Fe3+ used as enhanced electrochemical immunosensing substrate for tumor biomarker
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- Fabrication and characterization of novel macroporous Jeffamine/diamino hexane cryogels for enhanced Cu(II) metal uptake: Optimization, isotherms, kinetics and thermodynamic studies
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- Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by laccase from Trametes versicolor covalently immobilized on amino-functionalized SBA-15
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- Fabrication of Vertically aligned Copper Nanotubes as a Novel Electrode for Enzymatic Biofuel Cells
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- Two-dimensional infrared spectroscopic study on the thermally induced structural changes of glutaraldehyde-crosslinked collagen
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- Understanding of the Viscoelastic Response of the Human Corneal Stroma Induced by Riboflavin/UV-A Cross-Linking at the Nano Level
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- Non-toxic agarose/gelatin-based microencapsulation system containing gallic acid for antifungal application
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- Investigation of Phycobilisome Subunit Interaction Interfaces by Coupled Cross-linking and Mass Spectrometry
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- Protein products obtained by site‐preferred partial crosslinking in protein crystals and “liberated” by redissolution
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- Efficient asymmetric synthesis of d-N-formyl-phenylglycine via cross-linked nitrilase aggregates catalyzed dynamic kinetic resolution
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- Immobilization of (S)-mandelate dehydrogenase and its catalytic performance on stereoselective transformation of mandelic acid
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- Novel support for enzyme immobilization prepared by chemical activation with cysteine and glutaraldehyde
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- Characterization, Analysis, and Application of Fabricated Fe3O4-Chitosan-Pectinase Nanobiocatalyst
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- Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core–shell silica nanospheres
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- Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy
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- Miniaturised enzymatic conductometric biosensor with Nafion membrane for the direct determination of formaldehyde in water samples
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- Immobilization of defined laccase combinations for enhanced oxidation of phenolic contaminants
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- Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits
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- Biofunctional Phosphorylated Chitosan Hydrogels Prepared Above pH 6 and Effect of Crosslinkers on Gel Properties Towards Biomedical Applications
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- Effects of crosslinking on the mechanical properties, drug release and cytocompatibility of protein polymers
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- Co-immobilization of PEGylated Aspergillus flavipes l-methioninase with glutamate dehydrogenase: A novel catalytically stable anticancer consortium
  Enzyme and Microbial Technology, Vol. 54
- Efficient transformation of phenyl urea herbicide chloroxuron by laccase immobilized on zein polyurethane nanofiber
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- Development of a novel biosensor based on a conducting polymer
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- Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform
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- Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization
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- Highly emissive and biocompatible dopamine-derived oligomers as fluorescent probes for chemical detection and targeted bioimaging
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- A catalase-magnetic switch for cell proliferation
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- Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools
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- Structure and kinetics of chemically cross-linked protein gels from small-angle X-ray scattering
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- Preparation and Characterization of Cross-Linked Enzyme Aggregates of Amyloglucosidase
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- Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays
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- Highly Efficient Method Towards In Situ Immobilization of Invertase Using Cryogelation
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- Hybrid cellulase aggregate with a silica core for hydrolysis of cellulose and biomass
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- Immobilization of formate dehydrogenase from Candida boidinii through cross-linked enzyme aggregates
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- Continuous production of xylooligosaccharides in a packed bed reactor with immobilized–stabilized biocatalysts of xylanase from Aspergillus versicolor
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- Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release
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- Study on capillaries covalently bound with phospholipid vesicles for open‐tubular CEC and application to on‐line open‐tubular CEC‐MS
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- Internal Water and Microsecond Dynamics in Myoglobin
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- Antibodies in research of thymosin β4: investigation of cross‐reactivity and influence of fixatives
  10 October 2012 | Annals of the New York Academy of Sciences, Vol. 1270, No. 1
- Modified in situ and self-catalytic growth method for fabrication of Ag-coated nanocomposites with tailorable optical properties
  7 August 2012 | Journal of Nanoparticle Research, Vol. 14, No. 9
- Physicochemical Modification of Kafirin Microparticles and Their Ability To Bind Bone Morphogenetic Protein-2 (BMP-2), for Application as a Biomaterial
  17 August 2012 | Journal of Agricultural and Food Chemistry, Vol. 60, No. 34
- Autonomic Hydrogels through Postfunctionalization of Gelatin
  31 July 2012 | Chemistry of Materials, Vol. 24, No. 15
- Synthesis and characterization of a novel fish scale-immobilized chitosan adsorbent—Preliminary features of dichlorophenol sorption by solution calorimetry
  Journal of Hazardous Materials, Vol. 229-230
- Immobilization of thermostable β-glucosidase variants on acrylic supports for biocatalytic processes in hot water
  Journal of Molecular Catalysis B: Enzymatic, Vol. 80
- Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica
  Process Biochemistry, Vol. 47, No. 8
- Investigation of the binding of dioxin selective pentapeptides to a polyaniline matrix
  Synthetic Metals, Vol. 162, No. 13-14
- Structural Mechanism for Alteration of Collagen Gel Mechanics by Glutaraldehyde Crosslinking
  21 March 2012 | Connective Tissue Research, Vol. 53, No. 4
- Biofunctionalization and self-interaction chromatography in PDMS microchannels
  Biochemical Engineering Journal, Vol. 67
- Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids
  Biochemical Engineering Journal, Vol. 67
- Immobilized heterologous Rhizopus oryzae lipase: A feasible biocatalyst for the production of human milk fat substitutes
  Biochemical Engineering Journal, Vol. 67
- Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles
  21 June 2012 | Journal of Agricultural and Food Chemistry, Vol. 60, No. 26
- Continuous synthesis of l-malic acid using whole-cell microreactor
  Process Biochemistry, Vol. 47, No. 7
- Crosslinked aggregates of Rhizopus oryzae lipase as industrial biocatalysts: Preparation, optimization, characterization, and application for enantioselective resolution reactions
  26 June 2012 | Biotechnology Progress, Vol. 28, No. 4
- Amino acid-mediated aldolase immobilisation for enhanced catalysis and thermostability
  9 February 2012 | Bioprocess and Biosystems Engineering, Vol. 35, No. 5
- α-Amylase immobilization on gelatin: Optimization of process variables
  Journal of Genetic Engineering and Biotechnology, Vol. 10, No. 1
- Immobilization of a saccharifying raw starch hydrolyzing enzyme on functionalized and non-functionalized sepa beads
  Journal of Molecular Catalysis B: Enzymatic, Vol. 78
- Dimerization of ZIP promotes its transcriptional repressive function and biological activity
  The International Journal of Biochemistry & Cell Biology, Vol. 44, No. 6
- Atomic force microscopy‐based force spectroscopy — biological and biomedical applications
  2 May 2012 | IUBMB Life, Vol. 64, No. 6
- Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2 : Design of a heterogeneous D ‐amino acid oxidase catalyst on porous glass
  17 January 2012 | Biotechnology and Bioengineering, Vol. 109, No. 6
- Poly(glutaraldehyde)‐stabilized fish scale fibrillar collagen–some features of a new material for heavy metal sorption
  4 November 2011 | Journal of Applied Polymer Science, Vol. 124, No. 4
- Effect of pH on Protein Distribution in Electrospun PVA/BSA Composite Nanofibers
  18 April 2012 | Biomacromolecules, Vol. 13, No. 5
- A novel immobilization method for nuclease P1 on macroporous absorbent resin with glutaraldehyde cross-linking and determination of its properties
  Process Biochemistry, Vol. 47, No. 4
- Gold‐Decorated Carbon Composite Electrodes for Enzymatic Oxygen Reduction
  17 February 2012 | Electroanalysis, Vol. 24, No. 4
- The use of solution microcalorimetry to evaluate chemically modified fish scales as a viable adsorbent for heavy metals
  4 May 2011 | Journal of Thermal Analysis and Calorimetry, Vol. 107, No. 3
- Hydrazone self-crosslinking of multiphase elastin-like block copolymer networks
  Acta Biomaterialia, Vol. 8, No. 3
- Identification of a lipid‐related peak set to enhance the interpretation of TOF‐SIMS data from model and cellular membranes
  30 June 2011 | Surface and Interface Analysis, Vol. 44, No. 3
- A contact lens with an integrated lactate sensor
  Sensors and Actuators B: Chemical, Vol. 162, No. 1
- Investigation of a Biosensor Based on Phenylalanine Dehydrogenase Immobilized on a Polymer‐Blend Film for Phenylketonuria Diagnosis
  16 January 2012 | Electroanalysis, Vol. 24, No. 2
- 9.15 Industrial Applications of Asymmetric Synthesis using Cross-Linked Enzyme Aggregates
- Immobilized lipase on magnetic chitosan microspheres for transesterification of soybean oil
  Biomass and Bioenergy, Vol. 36
- Enzyme immobilization on epoxy supports in reverse micellar media: Prevention of enzyme denaturation
  Journal of Molecular Catalysis B: Enzymatic, Vol. 74, No. 1-2
- Crosslinked redox polymer enzyme electrodes containing carbon nanotubes for high and stable glucose oxidation current
  Physical Chemistry Chemical Physics, Vol. 14, No. 42
- Biocompatible magnetic cellulose–chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization
  Journal of Materials Chemistry, Vol. 22, No. 30
- Preparation of Chitosan Beads for Trypsin Immobilization
  16 April 2014 | Biotechnology & Biotechnological Equipment, Vol. 26, No. sup1
- Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein with bovine serum albumin and their immobilization for optical pH sensing
  Sensors and Actuators B: Chemical, Vol. 161, No. 1
- Improvement in Water Stability and Other Related Functional Properties of Thin Cast Kafirin Protein Films
  15 November 2011 | Journal of Agricultural and Food Chemistry, Vol. 59, No. 23
- Thermo-sensitive amphiphilic block copolymer poly (styrene-b-N-isopropylacrylamide) with switchable catalytic activity immobilizing pectinase
  3 December 2011 | Biotechnology and Bioprocess Engineering, Vol. 16, No. 6
- Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)
  2 September 2011 | Applied Microbiology and Biotechnology, Vol. 92, No. 3
- Immobilization and stabilization of α-galactosidase on Sepabeads EC-EA and EC-HA
  International Journal of Biological Macromolecules, Vol. 49, No. 4
- FTIR-ATR characterization of free Rhizomucor meihei lipase (RML), Lipozyme RM IM and chitosan-immobilized RML
  Journal of Molecular Catalysis B: Enzymatic, Vol. 72, No. 3-4
- Adjacent assembly of self-assembled monolayers for the construction of selective bio-platforms
  Sensors and Actuators B: Chemical, Vol. 159, No. 1
- Comparison of covalent immobilization of amylase on polystyrene pellets with pentaethylenehexamine and pentaethylene glycol spacers
  Bioresource Technology, Vol. 102, No. 20
- Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids
  Catalysis Communications, Vol. 14, No. 1
- Swelling–shrinking behavior of chemically cross-linked polypeptide gels from poly(α-l-lysine), poly(α-dl-lysine), poly(ɛ-l-lysine) and thermally prepared poly(lysine): Effects of pH, temperature and additives in the solution
  Colloids and Surfaces B: Biointerfaces, Vol. 87, No. 2
- Amperometric tyrosinase based biosensor using an electropolymerized phosphate-doped polypyrrole film as an immobilization support. Application for detection of phenolic compounds
  Electrochimica Acta, Vol. 56, No. 24
- Development of films based on blends of gelatin and poly(vinyl alcohol) cross linked with glutaraldehyde
  Food Hydrocolloids, Vol. 25, No. 7
- Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes
  14 July 2011 | Advanced Synthesis & Catalysis, Vol. 353, No. 13
- Use of Engineered Unique Cysteine Residues to Facilitate Oriented Coupling of Proteins Directly to a Gold Substrate
  28 July 2011 | Photochemistry and Photobiology, Vol. 87, No. 5
- Visualizing Cell Extracellular Matrix (ECM) Deposited by Cells Cultured on Aligned Bacteriophage M13 Thin Films
  6 July 2011 | Langmuir, Vol. 27, No. 15
- Covalent Biofunctionalization of Cellulose Acetate with Thermostable Chimeric Avidin
  16 June 2011 | ACS Applied Materials & Interfaces, Vol. 3, No. 7
- Comparison of the Properties of Lipase Immobilized onto Mesoporous Resins by Different Methods
  13 January 2011 | Applied Biochemistry and Biotechnology, Vol. 164, No. 5
- Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests
  Colloids and Surfaces B: Biointerfaces, Vol. 84, No. 2
- Sucrose hydrolysis by invertase immobilized on functionalized porous silicon
  Journal of Molecular Catalysis B: Enzymatic, Vol. 69, No. 3-4
- Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform
  7 March 2011 | Biotechnology Journal, Vol. 6, No. 5
- A facile technique to prepare cross-linked enzyme aggregates using p-benzoquinone as cross-linking agent
  28 March 2011 | Korean Journal of Chemical Engineering, Vol. 28, No. 4
- Synthesis and Properties of Immobilized Pectinase onto the Macroporous Polyacrylamide Microspheres
  22 February 2011 | Journal of Agricultural and Food Chemistry, Vol. 59, No. 6
- Immobilization of horseradish peroxidase as crosslinked enzyme aggregates (CLEAs)
  Process Biochemistry, Vol. 46, No. 3
- Immobilization of organophosphate hydrolase on an amyloid fibril nanoscaffold: Towards bioremediation and chemical detoxification
  23 November 2010 | Biotechnology Progress, Vol. 27, No. 2
- Immobilization of naringinase on mesoporous molecular sieve MCM-41 and its application to debittering of white grapefruit
  Applied Surface Science, Vol. 257, No. 9
- TMEM16A(a)/anoctamin-1 Shares a Homodimeric Architecture with CLC Chloride Channels
  Molecular & Cellular Proteomics, Vol. 10, No. 2
- Cross-Linked Enzyme Aggregates as Industrial Biocatalysts
  7 December 2010 | Organic Process Research & Development, Vol. 15, No. 1
- Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy
  28 January 2011
- Stable and efficient immobilization technique of aldolase under consecutive microwave irradiation at low temperature
  Bioresource Technology, Vol. 102, No. 2
- Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester
  Bioresource Technology, Vol. 102, No. 2
- The respiratory allergen glutaraldehyde in the local lymph node assay: Sensitization by skin exposure, but not by inhalation
  Toxicology, Vol. 279, No. 1-3
- Covalent binding of phospholipid vesicles on fused silica capillaries for electrochromatography
  Soft Matter, Vol. 7, No. 13
- Production of new immobilized β-glucosidase preparation for food industrial purposes by using kinetic desorption method
  Acta Alimentaria, Vol. 40, No. Supplement 1
- Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application
  International Journal of Electrochemistry, Vol. 2011
- Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detection
  Biosensors and Bioelectronics, Vol. 26, No. 5
- Biomimetic biosensor based on lipidic layers containing tyrosinase and lutetium bisphthalocyanine for the detection of antioxidants
  Biosensors and Bioelectronics, Vol. 26, No. 5
- Biocatalytic nylon nanofibrous membranes
  15 October 2010 | Analytical and Bioanalytical Chemistry, Vol. 398, No. 7-8
- Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase
  Journal of Biotechnology, Vol. 150, No. 4
- Aspergillus sp. lipase: Potential biocatalyst for industrial use
  Journal of Molecular Catalysis B: Enzymatic, Vol. 67, No. 3-4
- Improved Immobilization Supports for Candida Antarctica Lipase B
  25 November 2010
- Cross‐Linked Enzyme Aggregates as Industrial Biocatalysts
  5 October 2010
- Merging Molecular Electron Microscopy and Mass Spectrometry by Carbon Film-assisted Endoproteinase Digestion
  Molecular & Cellular Proteomics, Vol. 9, No. 8
- Synthesis and characteristics of cryogels of chitosan crosslinked by glutaric aldehyde
  3 September 2010 | Polymer Science Series A, Vol. 52, No. 8
- Novel ELISAs for Screening of the Biogenic Amines GABA, Glycine, β-Phenylethylamine, Agmatine, and Taurine Using One Derivatization Procedure of Whole Urine Samples
  29 June 2010 | Analytical Chemistry, Vol. 82, No. 15
- Construction and operation of a fibrous bed reactor with immobilized lactonase for efficient production of (R)-α-hydroxy-γ-butyrolactone
  Biochemical Engineering Journal, Vol. 50, No. 1-2
- Chemical modification and immobilisation of laccase from Trametes hirsuta and from Myceliophthora thermophila
  Enzyme and Microbial Technology, Vol. 46, No. 6
- Master equation approach to single oligomeric enzyme catalysis: Mechanically controlled further catalysis
  5 April 2010 | The Journal of Chemical Physics, Vol. 132, No. 13
- Mechanism of 1 H– 14 N cross-relaxation in immobilized proteins
  Journal of Magnetic Resonance, Vol. 203, No. 2
- Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone
  Bioresource Technology, Vol. 101, No. 6
- Covalent immobilization of invertase on polyurethane, plast-film and ferromagnetic Dacron
  Bioresource Technology, Vol. 101, No. 6
- Surface functionalization of silica-coated magnetic nanoparticles for covalent attachment of cholesterol oxidase
  Journal of Magnetism and Magnetic Materials, Vol. 322, No. 2
- Immobilization of l-lactate dehydrogenase on magnetic nanoclusters for chiral synthesis of pharmaceutical compounds
  Biochemical Engineering Journal, Vol. 48, No. 1
- Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
  Enzyme and Microbial Technology, Vol. 45, No. 6-7
- The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment
  Enzyme and Microbial Technology, Vol. 45, No. 6-7
- Novel and efficient method for immobilization and stabilization of β-d-galactosidase by covalent attachment onto magnetic Fe3O4–chitosan nanoparticles
  Journal of Molecular Catalysis B: Enzymatic, Vol. 61, No. 3-4
- Evaluation of cross-linked aggregates from purified Bacillus subtilislevansucrase mutants for transfructosylation reactions
  27 July 2009 | BMC Biotechnology, Vol. 9, No. 1
- Immobilization of alliinase with a water soluble–insoluble reversible N-succinyl-chitosan for allicin production
  Enzyme and Microbial Technology, Vol. 45, No. 4
- Effect of the glutaraldehyde derivatives of Calix[n]arene as cross-linker reagents on lipase immobilization
  22 March 2009 | Journal of Inclusion Phenomena and Macrocyclic Chemistry, Vol. 64, No. 3-4
- Osmolyte perturbation reveals conformational equilibria in spin‐labeled proteins
  7 July 2009 | Protein Science, Vol. 18, No. 8
- Nanocomposite based on modified TiO2–BSA for functional applications
  Colloids and Surfaces B: Biointerfaces, Vol. 71, No. 2
- Synthesis of calix[n]arene-based silica polymers for lipase immobilization
  Journal of Molecular Catalysis B: Enzymatic, Vol. 58, No. 1-4
- Immobilization of HRP in Mesoporous Silica and Its Application for the Construction of Polyaniline Modified Hydrogen Peroxide Biosensor
  12 June 2009 | Sensors, Vol. 9, No. 6
- Bioconjugation techniques for microfluidic biosensors
  12 March 2009 | Analytical and Bioanalytical Chemistry, Vol. 394, No. 2
- Synthesis and characterization of cyclodextrin-based polymers as a support for immobilization of Candida rugosa lipase
  Journal of Molecular Catalysis B: Enzymatic, Vol. 57, No. 1-4
- About mechanism of chitosan cross-linking with glutaraldehyde
  26 May 2009 | Russian Journal of Bioorganic Chemistry, Vol. 35, No. 3
- Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes
  6 March 2009 | Biomacromolecules, Vol. 10, No. 4
- The single NTPase gene of Drosophila melanogaster encodes an intracellular nucleoside triphosphate diphosphohydrolase 6 (NTPDase6)
  Archives of Biochemistry and Biophysics, Vol. 484, No. 1
- Synthesis of a glutaraldehyde derivative of calix[4]arene as a cross-linker reagent for lipase immobilization
  10 September 2008 | Journal of Inclusion Phenomena and Macrocyclic Chemistry, Vol. 63, No. 1-2
- Maleimide conjugation markedly enhances the immunogenicity of both human and murine idiotype-KLH vaccines
  Molecular Immunology, Vol. 46, No. 3
- Poly(vinyl alcohol) films crosslinked by glutaraldehyde under mild conditions
  11 December 2008 | Journal of Applied Polymer Science, Vol. 111, No. 6
- Investigation of Non-Nucleophilic Additives for the Reduction of Morphological Anomalies in Protein Arrays
  17 October 2008 | Langmuir, Vol. 24, No. 22
- Immobilized Enzymes in Organic Synthesis
  25 February 2009
- Sulfhydryl-Based Tumor Antigen-Carrier Protein Conjugates Stimulate Superior Antitumor Immunity against B Cell Lymphomas
  The Journal of Immunology, Vol. 181, No. 6
- Mechanical and chemical characteristics of an autologous glue
  13 December 2007 | Journal of Biomedical Materials Research Part A, Vol. 86A, No. 4
- Kinetic Controlled Synthesis of pH-Responsive Network Alginate
  31 July 2008 | Biomacromolecules, Vol. 9, No. 9
- Reductive Glutaraldehydation of Amine Groups for Identification of Protein N-termini
  18 July 2008 | Journal of Proteome Research, Vol. 7, No. 9
- Optimization of Biocatalyst Specific Activity for Glycolic Acid Production
  31 July 2008 | Advanced Synthesis & Catalysis, Vol. 350, No. 11-12
- Immobilization of β-galactosidase from Kluyveromyces lactis onto a polysiloxane–polyvinyl alcohol magnetic (mPOS–PVA) composite for lactose hydrolysis
  Catalysis Communications, Vol. 9, No. 14
- Tailored functionalization of low‐density polyethylene surfaces
  27 February 2008 | Journal of Applied Polymer Science, Vol. 108, No. 5
- A novel method for the immobilization of glucoamylase onto polyglutaraldehyde-activated gelatin
  Biochemical Engineering Journal, Vol. 39, No. 3
- Immobilization of Biological Catalysts
  15 March 2008
- Nanosecond to Microsecond Protein Dynamics Probed by Magnetic Relaxation Dispersion of Buried Water Molecules
  10 January 2008 | Journal of the American Chemical Society, Vol. 130, No. 5
- Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy
  Enzyme and Microbial Technology, Vol. 42, No. 3
- Preparation of cross-linked tyrosinase aggregates
  Process Biochemistry, Vol. 43, No. 2
- Mycobacterium tuberculosis CYP130
  Journal of Biological Chemistry, Vol. 283, No. 8
- GraFix: sample preparation for single-particle electron cryomicroscopy
  23 December 2007 | Nature Methods, Vol. 5, No. 1
- A method for lipase co-precipitation in a biodegradable protein matrix
  Biotechnology and Bioengineering, Vol. 98, No. 6
- Laccase immobilization on enzymatically functionalized polyamide 6,6 fibres
  Enzyme and Microbial Technology, Vol. 41, No. 6-7
- Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X‐ray structure analysis
  26 April 2007 | Biotechnology and Bioengineering, Vol. 98, No. 3
- Cloning and characterization of AtNUDT13, a novel mitochondrial Arabidopsis thaliana Nudix hydrolase specific for long‐chain diadenosine polyphosphates
  30 August 2007 | The FEBS Journal, Vol. 274, No. 18
- Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers
  Enzyme and Microbial Technology, Vol. 41, No. 3
- Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization
  Journal of Biotechnology, Vol. 131, No. 2
- Synthesis of an affinity adsorbent based on silica gel and its application in endotoxin removal
  Reactive and Functional Polymers, Vol. 67, No. 8
- Rice black-streaked dwarf virus outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution
  Virus Research, Vol. 127, No. 1
- Cotton fabric: A natural matrix suitable for controlled release systems
  Enzyme and Microbial Technology, Vol. 40, No. 7
- Improvement of enzyme activity, stability and selectivity via immobilization techniques
  Enzyme and Microbial Technology, Vol. 40, No. 6
- Nanowiring of the Catalytic Site of Novel Molecular Enzyme−Metal Hybrids to Electrodes
  28 March 2007 | The Journal of Physical Chemistry C, Vol. 111, No. 15
- Chitosan-immobilized lipases for the catalysis of fatty acid esterifications
  Enzyme and Microbial Technology, Vol. 40, No. 4
- Mechanisms of Action
  27 February 2007
- Crosslinked enzyme aggregates in hierarchically‐ordered mesoporous silica: A simple and effective method for enzyme stabilization
  19 September 2006 | Biotechnology and Bioengineering, Vol. 96, No. 2
- Single Polyelectrolyte Microcapsules Fabricated By Glutaraldehyde‐Mediated Covalent Layer‐By‐Layer Assembly
  1 December 2006 | Macromolecular Rapid Communications, Vol. 27, No. 24
- Different mechanisms of protein immobilization on glutaraldehyde activated supports: Effect of support activation and immobilization conditions
  Enzyme and Microbial Technology, Vol. 39, No. 4
- Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins
  Enzyme and Microbial Technology, Vol. 39, No. 2
- Hydrolysis of Maltoheptaose in Flow through Silicon Wafer Microreactors Containing Immobilised α‐Amylase and Glycoamylase
  2 May 2006 | Starch - Stärke, Vol. 58, No. 5
- Aminopropargyl derivative of terpyridine-bis(methyl-enamine) tetraacetic acid chelate of europium (Eu (TMT)-AP3): a new reagent for fluorescent labelling of proteins and peptides
  Organic & Biomolecular Chemistry, Vol. 4, No. 22
- A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica
  28 October 2005 | Small, Vol. 1, No. 12
- Cloning and characterization of an Arabidopsis thaliana Nudix hydrolase homologous to the mammalian GFG protein
  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1752, No. 2
- Some special features of glyoxyl supports to immobilize proteins
  Enzyme and Microbial Technology, Vol. 37, No. 4
- The Properties of Covalently Immobilized Trypsin on Soap‐Free P(MMA‐EA‐AA) Latex Particles
  8 April 2005 | Macromolecular Bioscience, Vol. 5, No. 4

Vol. 37, No. 5

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Published online 6 June 2018

Published in print November 2004

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Competing Interests Statement

The authors declare no competing interests.

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