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*Author for correspondence: Tel.: +1 617 992 3270;

E-mail Address: padmanabhan.eangoor@merck.com

https://orcid.org/0000-0001-8253-2411

Pharmacokinetics, Pharmacodynamics & Drug Metabolism, Merck & Co., 33 Avenue Louis Pasteur, Boston, MA 02115, USA

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Sharmistha Das

Pharmacokinetics, Pharmacodynamics & Drug Metabolism, Merck & Co., 33 Avenue Louis Pasteur, Boston, MA 02115, USA

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Vincenzo Pucci

Pharmacokinetics, Pharmacodynamics & Drug Metabolism, Merck & Co., 33 Avenue Louis Pasteur, Boston, MA 02115, USA

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Published Online:1 Dec 2020https://doi.org/10.4155/bio-2020-0258

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Abstract

Drug discovery is moving at a rapid pace and a fast turnaround of bioanalytical data is needed to sustain this pace. This article focuses on the evaluation of time-saving homogeneous proximity immunoassays such as Amplified Luminescent Proximity Homogeneous Assay, Time-Resolved Fluorescence Resonance Energy Transfer and Spatial Proximity Analyte Reagent Capture Luminescence as an alternative to industry popular platforms like mesoscale discovery (MSD) and Gyrolab^®. Our evaluation showed that no one platform can be considered the best for all the parameters assessed. Homogeneous proximity platforms were found to be advantageous over MSD and Gyrolab for certain applications and are herein discussed. The factors affecting the performance of homogeneous assays and appropriate corrections are discussed. The homogeneous assays, due to their flexibility, hold a lot of untapped potential for the future of bioanalysis.

Keywords:

Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 12, No. 24

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History

Received 29 September 2020

Accepted 26 October 2020

Published online 1 December 2020

Published in print December 2020

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Keywords

Acknowledgments

The authors would like to sincerely thank H Tang for reviewing the manuscript and providing valuable feedback.

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

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Evaluation of homogeneous proximity immunoassays for preclinical bioanalysis

Abstract

References