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Preliminary Communication

Solid-phase microextraction for assessment of plasma protein binding, a complement to rapid equilibrium dialysis

    Sheelan Ahmad

    *Author for correspondence:

    E-mail Address: Sheelan.2.Ahmad@gsk.com

    Research & Development, GlaxoSmithKline, Stevenage, UK

    Department of Clinical, School of Life & Medical Sciences, Pharmaceutical & Biological Sciences, University of Hertfordshire, Hatfield, UK

    ,
    Daniel Baker

    Department of Clinical, School of Life & Medical Sciences, Pharmaceutical & Biological Sciences, University of Hertfordshire, Hatfield, UK

    ,
    Darragh Murnane

    Department of Clinical, School of Life & Medical Sciences, Pharmaceutical & Biological Sciences, University of Hertfordshire, Hatfield, UK

    ,
    Neil Spooner

    Department of Clinical, School of Life & Medical Sciences, Pharmaceutical & Biological Sciences, University of Hertfordshire, Hatfield, UK

    Spooner Bioanalytical Solutions Ltd., Hertford, UK

    &
    Ute Gerhard

    Department of Clinical, School of Life & Medical Sciences, Pharmaceutical & Biological Sciences, University of Hertfordshire, Hatfield, UK

    Published Online:https://doi.org/10.4155/bio-2021-0109

    Aim: Determination of plasma protein binding (PPB) is considered vital for better understanding of pharmacokinetic and pharmacodynamic activities of drugs due to the role of free concentration in pharmacological response. Methodology & results: Solid-phase microextraction (SPME) was investigated for measurement of PPB from biological matrices and compared with a gold standard approach (rapid equilibrium dialysis [RED]). Discussion & conclusion: SPME-derived values of PPB correlated well with literature values, and those determined by RED. Respectively, average protein binding across three concentrations by RED and SPME was 33.1 and 31.7% for metoprolol, 89.0 and 86.6% for propranolol and 99.2 and 99.0% for diclofenac. This study generates some evidence for SPME as an alternative platform for the determination of PPB.

    Graphical abstract

    Papers of special note have been highlighted as: • of interest

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