Research Article

A rapid multidimensional GC–flame-ionization detector method for determination of fatty acid methyl esters

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Key Laboratory of Insect Development & Evolutionary Biology, Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, NO 300 Fenglin Road, Shanghai 200023, People’s Republic of China.

Department of Epidemiology & Public Health, Yong Loo Lin School of Medicine, National University of Singapore. 11759, Singapore

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Email the corresponding author at yjxutju@gmail.com

Jianbing Zhang

Department of Epidemiology & Public Health, Yong Loo Lin School of Medicine, National University of Singapore. 11759, Singapore

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Published Online:25 Feb 2014https://doi.org/10.4155/bio.13.305

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Abstract

Background: Fatty acids play important roles in the pathogenesis of many diseases. In order to investigate a wide spectrum of fatty acids in biological matrix, we developed an approach for quantitative analysis of fatty acids based on a heart-cutting 2D gas chromatographic-flame ionization detector system. Results: This system uses a Dean Switch interface between the primary polar and secondary nonpolar capillary column. Most of the standards were separated by the primary column. Unresolved components are selectively transferred to the secondary column, where they are completely separated. This method was further evaluated using plasma obtained from 11 healthy and 11 chronic coronary artery disease patients. Conclusion: Our results prove that this approach is sensitive, precise and specific, capable of measuring 37 specific fatty acids. It indicates that this approach offers a reliable and sensitive technical platform for comprehensive quantification of fatty acids.

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Published online 25 February 2014

Published in print February 2014

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Financial & competing interests disclosure

This study was supported by the Open Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University (No. SKLNMKF201304). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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