The performance of five different dried blood spot cards for the analysis of six immunosuppressants
Abstract
Background: The relation between hematocrit, substance concentration, extraction recovery and spot formation of tacrolimus, sirolimus, everolimus, ascomycin, temsirolimus and cyclosporin A was investigated for Whatman 31 ET CHR, Whatman FTA DMPK-C, Whatman 903, Perkin Elmer 226 and Agilent Bond Elut DMS DBS cards. Results & Discussion: We found that all DBS cards showed the same hematocrit and concentration-dependent recovery patterns for sirolimus, everolimus and temsirolimus. At high concentrations, the total hematocrit effects were much more pronounced than at low concentrations for tacrolimus, sirolimus, everolimus, ascomycin and temsirolimus. Conclusion: The tested card types showed differences in performance, especially at extreme concentrations and hematocrit values. It may be useful to investigate the performance of different types of DBS cards prior to analytical method validation.
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References
- 1 . Dried blood spot methods in therapeutic drug monitoring: methods, assays, and pitfalls. Ther. Drug Monit. 31(3), 327–336 (2009).
- 2 . Dried blood spot sampling in combination with LC–MS/MS for quantitative analysis of small molecules. Biomed. Chromatogr. 24(1), 49–65 (2010).
- 3 . Therapeutic drug monitoring by dried blood spot: progress to date and future directions. Clin. Pharmacokinet. 53(11), 961–973 (2014).
- 4 . Fast LC–MS/MS analysis of tacrolimus, sirolimus, everolimus and cyclosporin A in dried blood spots and the influence of the hematocrit and immunosuppressant concentration on recovery. Talanta 115, 47–54 (2013).• Showed that the hematocrit (HT) does not only affect spot size but also affects the dried blood spot recovery of immunosuppressants.
- 5 . Determination of moxifloxacin in dried blood spots using LC–MS/MS and the impact of the hematocrit and blood volume. J. Chromatogr. B. Analyt Technol. Biomed. Life Sci. 879(15–16), 1063–1070 (2011).
- 6 . Analysis of cyclosporin A, tacrolimus, sirolimus, and everolimus in dried blood spot samples using liquid chromatography tandem mass spectrometry. Anal. Bioanal Chem. 404(6–7), 1803–1811 (2012).
- 7 The relation of the number of hydrogen bond acceptorswith recoveries of immunosuppressants in dried blood spot analysis. Bioanalysis (2015)(In Press).
- 8 National Center for Biotechnology Information. PubChem Compound Database: CID=6473866; CID=5284616; CID=6442177; CID=5284373. www.ncbi.nlm.nih.gov/pccompound
- 9 . Use of filter paper for the collection and analysis of human whole blood specimens. J. Nutr. 131(5), S1631–S1636 (2001).
- 10 . Performance properties of filter paper devices for whole blood collection. Bioanalysis 2(8), 1397–1403 (2010).
- 11 . The effect of hematocrit and punch location on assay bias during quantitative bioanalysis of dried blood spot samples. Bioanalysis 3(20), 2335–2347 (2011).
- 12 Agilent Technologies. www.chem.agilent.com
- 13 What is the right blood hematocrit preparation procedure for standards and quality control samples for dried blood spot analysis? Bioanalysis 7(3), 345–351 (2015).• Showed the correct HT preparation procedure for dried blood spot validation and sample analysis.
