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Research Article

Overall biochemical changes in bacteria photosensitized with cationic porphyrins monitored by infrared spectroscopy

    Eliana Alves‡

    Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

    ‡Authors contributed equally

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    ,
    Catarina Moreirinha‡

    Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

    ‡Authors contributed equally

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    ,
    Maria AF Faustino

    Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

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    ,
    Ângela Cunha

    Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

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    ,
    Ivonne Delgadillo

    *Author for correspondence:

    E-mail Address: ivonne@ua.pt

    Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

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    ,
    Maria GPMS Neves

    Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

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    &
    Adelaide Almeida**

    Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810–193 Aveiro, Portugal

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    Published Online:13 Apr 2016https://doi.org/10.4155/fmc-2015-0008

    Abstract

    Background. Photodynamic inactivation of micro-organisms is a promising nonantibiotic multitarget approach to treat localized and superficial infections through oxidative stress. Herein, the changes occurring on major cellular components of Escherichia coli and Staphylococcus warneri, induced by photosensitization with cationic porphyrins (Tri-Py+-Me-PF and Tetra-Py+-Me) and white light, were monitored by infrared spectroscopy. Results: In E. coli, most of the changes occurred on proteins and lipids, suggesting a key effect on lipopolysaccharides in the first irradiation times. In S. warneri, proteins were the major molecular targets of oxidative damage but phospholipids and polysaccharides were also affected. Conclusion: Infrared spectroscopy is a very interesting tool to monitor biochemical changes induced by photosensitization in bacteria and also to infer on its mechanism of action.

    Papers of special note have been highlighted as: • of interest

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