Versatile tools of synthetic biology applied to drug discovery and production

References

• 1. Traykovska M, Miedema S, Penchovsky R. Clinical trials of functional nucleic acids: antisense oligonucleotides and aptamers. Int. Journal of Biomedical and Clinical Engineering (IJBCE) 7(2), 46–60 (2018).
  Google Scholar
• 2. Penchovsky R, Birch-Hirschfeld E, McCaskill JS. End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads. Nucleic Acids Res. 28(22), e98 (2000).
  Medline CASGoogle Scholar
• 3. Penchovsky R. Engineering integrated digital circuits with allosteric ribozymes for scaling up molecular computation and diagnostics. ACS Synth. Biol. 1(10), 471–482 (2012).
  Medline CASGoogle Scholar
• 4. Chappell J, Watters KE, Takahashi MK, Lucks JB. A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future. Curr. Opin. Chem. Biol. 28, 47–56 (2015).
  Medline CASGoogle Scholar
• 5. Hollywood KA, Schmidt K, Takano E, Breitling R. Metabolomics tools for the synthetic biology of natural products. Curr. Opin. Biotechnol. 54, 114–120 (2018).
  Medline CASGoogle Scholar
• 6. Liu D, Pakrasi HB. Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803. Microb. Cell Fact. 17(1), 48 (2018).
  MedlineGoogle Scholar
• 7. MacDonald IC, Deans TL. Tools and applications in synthetic biology. Adv. Drug Deliv. Rev. 105(Pt A), 20–34 (2016).
  Medline CASGoogle Scholar
• 8. Alnasser SM. Review on mechanistic strategy of gene therapy in the treatment of disease. Gene 769, 145246 (2021).
  Medline CASGoogle Scholar
• 9. Kopka J, Fernie AR. Editorial overview: plant synthetic and systems biology. Curr. Opin. Biotechnol. 49, viii–xi (2018).
  MedlineGoogle Scholar
• 10. Jagadevan S, Banerjee A, Banerjee C et al. Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production. Biotechnology for Biofuels 11, 185 (2018).
  MedlineGoogle Scholar
• 11. Cantelli CR, Dassonville-Klimpt A, Sonnet P. A review of current and promising nontuberculous mycobacteria antibiotics. Future Med. Chem. 13(16), 1367–1395 (2021).
  CASGoogle Scholar
• 12. Kaloudas D, Pavlova N, Penchovsky R. Lignocellulose, algal biomass, biofuels and biohydrogen: a review. Envt. Chem. Lett. 19(4), 2809–2824 (2021).
  CASGoogle Scholar
• 13. Buddingh BC, van Hest JCM. Artificial cells: synthetic compartments with life-like functionality and adaptivity. Acc. Chem. Res. 50(4), 769–777 (2017).
  Medline CASGoogle Scholar
• 14. Elani Y. Interfacing living and synthetic cells as an emerging frontier in synthetic biology. Angewandte Chemie International Edition 60(11), 5602–5611 (2021).
  Medline CASGoogle Scholar
• 15. Penchovsky R, Breaker RR. Computational design and experimental validation of oligonucleotide-sensing allosteric ribozymes. Nat. Biotechnol. 23(11), 1424–1433 (2005).
  Medline CASGoogle Scholar
• 16. Takahashi MK, Tan X, Dy AJ et al. A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers. Nat. Commun. 9(1), 3347 (2018).
  MedlineGoogle Scholar
• 17. Liu C. Liability for transboundary damage of genetically modified organisms: existing patterns and application. Beijing Law Review 12(1), 11 (2021).
  CASGoogle Scholar
• 18. Deckers M, Deforce D, Fraiture MA, Roosens NHC. Genetically modified micro-organisms for industrial food enzyme production: an overview. Foods 9(3), 326–346 (2020).
  CASGoogle Scholar
• 19. Penchovsky R, Stoilova CC. Riboswitch-based antibacterial drug discovery using high-throughput screening methods. Expert Opin. Drug Discov. 8(1), 65–82 (2013).
  Medline CASGoogle Scholar
• 20. Deepika M, Sumathy J. Production of BioBricks using microbes. Int. J. Curr. Res. Multi. (IJCRM) 5(1), 13–27 (2020).
  Google Scholar
• 21. Staal J, Alci K, Schamphelaire WD, Vanhoucke M, Beyaert R. Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site. BioTechniques 66(6), 254–259 (2019).
  CASGoogle Scholar
• 22. Van der Weyden L, Jonkers J, Adams DJ. The use of CRISPR/CAS9-based gene editing strategies to explore cancer gene function in mice. Curr. Op. Gen. & Dev. 66, 57–62 (2021).
  MedlineGoogle Scholar
• 23. Liang Z, Qin Z, Riker AI, Xi Y. CRISPR/CAS9 ablating viral microRNA promotes lytic reactivation of Kaposi's sarcoma-associated herpesvirus. Biochem. Biophys. Res. Commun. 533(4), 1400–1405 (2020).
  Medline CASGoogle Scholar
• 24. Ghaemi A, Bagheri E, Abnous K, Taghdisi SM, Ramezani M, Alibolandi M. CRISPR-CAS9 genome editing delivery systems for targeted cancer therapy. Life Sci. 267, 118969 (2021).
  Medline CASGoogle Scholar
• 25. Hartz P, Gehl M, Konig L, Bernhardt R, Hannemann F. Development and application of a highly efficient CRISPR-CAS9 system for genome engineering in Bacillus megaterium. J. Biotechnol. (2021).
  MedlineGoogle Scholar
• 26. Sharma G, Sharma AR, Bhattacharya M, Lee SS, Chakraborty C. CRISPR-CAS9: a preclinical and clinical perspective for the treatment of human diseases. Mol. Ther. 29(2), 571–586 (2021).
  Medline CASGoogle Scholar
• 27. Rainha J, Rodrigues JL, Rodrigues LR. CRISPR-CAS9: a powerful tool to efficiently engineer Saccharomyces cerevisiae. Life (Basel) 11(1), 1–16 (2020).
  MedlineGoogle Scholar
• 28. Kumar SA, Kumar TDA, Beeraka NM et al. Machine learning and deep learning in data-driven decision making of drug discovery and challenges in high-quality data acquisition in the pharmaceutical industry. Future Med. Chem. 14(4), 245–270 (2022).
  CASGoogle Scholar
• 29. Dwivedi A, Kumar K, Verma PK. Chapter 4 – constructing synthetic pathways in plants: strategies and tools. In: Current Developments in Biotechnology and Bioengineering. Singh SPPandey ADu GKumar S (Eds). Elsevier, USA, 56–77 (2019).
  Google Scholar
• 30. Gandhi SG. Chapter 8 – synthetic biology for production of commercially important natural product small molecules. In: Current Developments in Biotechnology and Bioengineering. Singh SPPandey ADu GKumar S (Eds). Elsevier, USA, 120–138 (2019).
  Google Scholar
• 31. An N, Liu S, Ding H, Wang H, Zhang L, Alterovitz G. A data-driven method for BioBrick quality assessment. 2021 IEEE 2nd International Conference on Big Data, Artificial Intelligence and Internet of Things Engineering (ICBAIE). 20–30 (2021).
  Google Scholar
• 32. Paul PEVViswanath B (Eds). Chapter 22 – Recent trends in the development of high-performance microbial cell factories for production of bio-based chemicals. In :Recent Developments in Applied Microbiology and Biochemistry. Academic Press, USA, 241–246 (2021).
  Google Scholar
• 33. Johnson J, Harman VM, Franco C et al. Synthetic biology meets proteomics: construction of à la carte QconCATs for absolute protein quantification. bioRxiv 2021.2004.2013.439592 (2021).
  Google Scholar
• 34. Fossati E, Narcross L, Ekins A, Falgueyret JP, Martin VJ. Synthesis of morphinan alkaloids in Saccharomyces cerevisiae. PLOS ONE 10(4), e0124459 (2015).
  MedlineGoogle Scholar
• 35. DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE. An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose. Nat. Chem. Biol. 11(7), 465–471 (2015).
  Medline CASGoogle Scholar
• 36. Zhou K, Qiao K, Edgar S, Stephanopoulos G. Distributing a metabolic pathway among a microbial consortium enhances production of natural products. Nat. Biotechnol. 33(4), 377–383 (2015).
  Medline CASGoogle Scholar
• 37. Dixit S, Shukla A, Singh V, Upadhyay SK. Engineering of plant metabolic pathway for nutritional improvement. In: Genome Engineering for Crop Improvement. Santosh Kumar Upadhyay (Ed.). USA, 351–379 (2021).
  Google Scholar
• 38. Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 12(2), 144–170 (2012).
  Medline CASGoogle Scholar
• 39. Beites T, Mendes M. Chassis optimization as a cornerstone for the application of synthetic biology based strategies in microbial secondary metabolism. Front. Microbiol. 6(906), 1–10 (2015).
  MedlineGoogle Scholar
• 40. Liu X, Cheng J, Zhang G et al. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches. Nat. Commun. 9(1), 448 (2018).
  MedlineGoogle Scholar
• 41. Alper HS, Avalos JL. Metabolic pathway engineering. Synth. Syst. Biotechnol. 3(1), 1–2 (2018).
  MedlineGoogle Scholar
• 42. Kawaguchi H, Yoshihara K, Hara KY, Hasunuma T, Ogino C, Kondo A. Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose. Microb. Cell Fact. 17(1), 76 (2018).
  MedlineGoogle Scholar
• 43. Wang H, Liu W, Shi F et al. Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli. Metab. Eng. 48, 279–287 (2018).
  Medline CASGoogle Scholar
• 44. Zhao M, Huang D, Zhang X, Koffas MAG, Zhou J, Deng Y. Metabolic engineering of Escherichia coli for producing adipic acid through the reverse adipate-degradation pathway. Metab. Eng. 47, 254–262 (2018).
  Medline CASGoogle Scholar
• 45. Nishimura Y, Matsui T, Ishii J, Kondo A. Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae. Microb. Cell Fact. 17(1), 38 (2018).
  MedlineGoogle Scholar
• 46. Ibdah M, Martens S, Gang DR. Biosynthetic pathway and metabolic engineering of plant dihydrochalcones. J. Agric. Food Chem. 66(10), 2273–2280 (2018).
  Medline CASGoogle Scholar
• 47. Gao C, Wang S, Hu G et al. Engineering Escherichia coli for malate production by integrating modular pathway characterization with CRISPRi-guided multiplexed metabolic tuning. Biotechnol. Bioeng. 115(3), 661–672 (2018).
  Medline CASGoogle Scholar
• 48. Traykovska M, Popova KB, Penchovsky R. Targeting glmS ribozyme with chimeric antisense oligonucleotides for antibacterial drug development. ACS Synthet. Bio. 10(11), 3167–3176 (2021).
  Medline CASGoogle Scholar
• 49. Pavlova N, Penchovsky R. Genome-wide bioinformatics analysis of FMN, SAM-I, glmS, TPP, lysine, purine, cobalamin, and SAH riboswitches for their applications as allosteric antibacterial drug targets in human pathogenic bacteria. Expert Opin. Ther. Targets 23(7), 631–643 (2019).
  Medline CASGoogle Scholar
• 50. Traykovska M, Penchovsky R. Engineering antisense oligonucleotides as antibacterial agents that target FMN riboswitches and inhibit the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli. ACS Synth. Biol. 1845–1855 (2022).
  MedlineGoogle Scholar
• 51. Xiao M, Lai W, Yu H et al. Assembly pathway selection with DNA reaction circuits for programming multiple cell–cell interactions. J. Am. Chem. Soc. 143(9), 3448–3454 (2021).
  Medline CASGoogle Scholar
• 52. Ding Y, Wu F, Tan C. Synthetic biology: a bridge between artificial and natural cells. Life (Basel) 4(4), 1092–1116 (2014).
  MedlineGoogle Scholar
• 53. Lu Y. Cell-free synthetic biology: engineering in an open world. Synth. Syst. Biotechnol. 2(1), 23–27 (2017).
  MedlineGoogle Scholar
• 54. Gibson DG, Glass JI, Lartigue C et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329(5987), 52–56 (2010).
  Medline CASGoogle Scholar
• 55. Hughes RA, Ellington AD. Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology. Cold Spring Harb. Perspect. Biol. 9(1), 1–17 (2017).
  Google Scholar
• 56. Glass JI, Merryman C, Wise KS, Hutchison CA 3rd, Smith HO. Minimal cells – real and imagined. Cold Spring Harb. perspect. Biol. 9(12), a023861 (2017).
  MedlineGoogle Scholar
• 57. Hutchison CA 3rd, Chuang RY, Noskov VN et al. Design and synthesis of a minimal bacterial genome. Science 351(6280), aad6253 (2016).
  MedlineGoogle Scholar
• 58. Luo Z, Yu K, Xie S et al. Compacting a synthetic yeast chromosome arm. Genome Biol. 22(1), 5 (2021).
  Medline CASGoogle Scholar
• 59. Boeke JD, Church G, Hessel A et al. Genome engineering. The Genome Project-Write. Science 353(6295), 126–127 (2016).
  Medline CASGoogle Scholar
• 60. Kamatani Y, Okada Y. Two decades after Human Genome Project: do large-genetic studies lead to path of the genomic medicine of complex diseases? J. Hum. Genet. 66(1), 1 (2021).
  MedlineGoogle Scholar
• 61. Gates AJ, Gysi DM, Kellis M, Barabasi AL. A wealth of discovery built on the Human Genome Project – by the numbers. Nature 590(7845), 212–215 (2021).
  Medline CASGoogle Scholar
• 62. Paik YK, Omenn GS, Thongboonkerd V, Marko-Varga G, Hancock WS. Genome-wide proteomics, Chromosome-Centric Human Proteome Project (C-HPP), part II. J. Proteome Res. 13(1), 1–4 (2014).
  Medline CASGoogle Scholar
• 63. Schindler D. Genetic engineering and synthetic genomics in yeast to understand life and boost biotechnology. Bioengineering (Basel, Switzerland) 7(4), 1–22 (2020).
  Google Scholar
• 64. Greene A, Pascarelli K, Broccoli D, Perkins E. Engineering synthetic chromosomes by sequential loading of multiple genomic payloads over 100 kilobase pairs in size. Molecular Therapy - Methods & Clinical Development 13, 463–473 (2019).
  Medline CASGoogle Scholar
• 65. Kung SH, Lund S, Murarka A, McPhee D, Paddon CJ. Approaches and recent developments for the commercial production of semi-synthetic artemisinin. Front. Plant Sci. 9, 87 (2018).
  MedlineGoogle Scholar
• 66. Hale V, Keasling JD, Renninger N, Diagana TT. Microbially derived artemisinin: a biotechnology solution to the global problem of access to affordable antimalarial drugs. Am. J. Trop. Med. Hyg. 77(Suppl. 6), S198–S202 (2007).
  MedlineGoogle Scholar
• 67. Winzeler EA, Manary MJ. Drug resistance genomics of the antimalarial drug artemisinin. Genome Biol. 15(11), 544 (2014).
  MedlineGoogle Scholar
• 68. Lopez C, Saravia C, Gomez A, Hoebeke J, Patarroyo MA. Mechanisms of genetically-based resistance to malaria. Gene 467(1-2), 1–12 (2010).
  Medline CASGoogle Scholar
• 69. Huang Q, Roessner CA, Croteau R, Scott AI. Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol. Bioorg. Med. Chem. 9(9), 2237–2242 (2001).
  Medline CASGoogle Scholar
• 70. Bian G, Yuan Y, Tao H et al. Production of taxadiene by engineering of mevalonate pathway in Escherichia coli and endophytic fungus Alternaria alternata TPF6. Biotechnol. J. 12(4), 1–10 (2017).
  CASGoogle Scholar
• 71. Kazanov MD, Vitreschak AG, Gelfand MS. Abundance and functional diversity of riboswitches in microbial communities. BMC Genomics 8, 347 (2007).
  MedlineGoogle Scholar
• 72. Malik S, Cusidó RM, Mirjalili MH, Moyano E, Palazón J, Bonfill M. Production of the anticancer drug taxol in Taxus baccata suspension cultures: a review. Process Biochemistry 46(1), 23–34 (2011).
  CASGoogle Scholar
• 73. Galanie S, Thodey K, Trenchard IJ, Filsinger Interrante M, Smolke CD. Complete biosynthesis of opioids in yeast. Science 349(6252), 1095–1100 (2015).
  Medline CASGoogle Scholar
• 74. Nakagawa A, Matsumura E, Koyanagi T et al. Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli. Nat. Commun. 7, 10390 (2016).
  Medline CASGoogle Scholar
• 75. Ramesh AM, Kesari V, Rangan L. Characterization of a stearoyl-acyl carrier protein desaturase gene from potential biofuel plant, Pongamia pinnata L. Gene 542(2), 113–121 (2014).
  Medline CASGoogle Scholar
• 76. Brooks SM, Alper HS. Applications, challenges, and needs for employing synthetic biology beyond the lab. Nature Communications 12(1), 1390 (2021).
  Medline CASGoogle Scholar
• 77. Voyvodic PL, Bonnet J. Cell-free biosensors for biomedical applications. Curr. Opin. Biomed. Engin. 13, 9–15 (2020).
  Google Scholar
• 78. Gräwe A, Dreyer A, Vornholt T et al. A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs. PLOS ONE 14(3), e0210940 (2019).
  MedlineGoogle Scholar
• 79. Duyen TT, Matsuura H, Ujiie K, Muraoka M, Harada K, Hirata K. Paper-based colorimetric biosensor for antibiotics inhibiting bacterial protein synthesis. J. Biosci. Bioengin. 123(1), 96–100 (2017).
  Medline CASGoogle Scholar
• 80. Voyvodic PL, Pandi A, Koch M et al. Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors. Nature Comm. 10(1), 1697 (2019).
  MedlineGoogle Scholar
• 81. Chen Z, He A, Liu Y, Huang W, Cai Z. Recent development on synthetic biological devices treating bladder cancer. Synth. Syst. Biotechnol. 1(4), 216–220 (2016).
  MedlineGoogle Scholar
• 82. Bak RO, Mikkelsen JG. miRNA sponges: soaking up miRNAs for regulation of gene expression. Wiley Interdiscip. Rev. RNA 5(3), 317–333 (2014).
  Medline CASGoogle Scholar
• 83. Tay FC, Lim JK, Zhu H, Hin LC, Wang S. Using artificial microRNA sponges to achieve microRNA loss-of-function in cancer cells. Adv. Drug Deliv. Rev. 81, 117–127 (2015).
  Medline CASGoogle Scholar
• 84. Karig DK. Cell-free synthetic biology for environmental sensing and remediation. Curr. Opin. Biotechnol. 45, 69–75 (2017).
  Medline CASGoogle Scholar
• 85. Pardee K, Green AA, Takahashi MK et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell 165(5), 1255–1266 (2016).
  Medline CASGoogle Scholar
• 86. Pardee K, Green AA, Ferrante T et al. Paper-based synthetic gene networks. Cell 159(4), 940–954 (2014).
  Medline CASGoogle Scholar
• 87. Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell 159(4), 925–939 (2014).
  Medline CASGoogle Scholar
• 88. Kim HJ, Jeong H, Lee SJ. Synthetic biology for microbial heavy metal biosensors. Analy. Bioanaly. Chem. 410(4), 1191–1203 (2018).
  Medline CASGoogle Scholar
• 89. Gutiérrez JC, Amaro F, Martín-González A. Heavy metal whole-cell biosensors using eukaryotic microorganisms: an updated critical review. Front. Microbiol. 6(48), 1–8 (2015).
  MedlineGoogle Scholar
• 90. Shetty RS, Deo SK, Liu Y, Daunert S. Fluorescence-based sensing system for copper using genetically engineered living yeast cells. Biotechnol. Bioeng. 88(5), 664–670 (2004).
  Medline CASGoogle Scholar
• 91. Park JN, Sohn MJ, Oh DB et al. Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems. Appl. Environ. Microbiol. 73(19), 5990–6000 (2007).
  Medline CASGoogle Scholar
• 92. Traykovska M, Popova KB, Penchovsky R. Targeting glmS ribozyme with chimeric antisense oligonucleotides for antibacterial drug development. ACS Synth. Biol. 10(11), 3167–3176 (2021).
  Medline CASGoogle Scholar
• 93. Kaloudas D, Pavlova N, Penchovsky R. EBWS: essential bioinformatics web services for sequence analyses. IEEE/ACM Trans. Comput. Biol. Bioinform. 16(3), 942–953 (2019).
  CASGoogle Scholar
• 94. Penchovsky R, Kostova GT. Computational selection and experimental validation of allosteric ribozymes that sense a specific sequence of human telomerase reverse transcriptase mRNAs as universal anticancer therapy agents. Nucleic Acid Ther. 23(6), 408–417 (2013).
  Medline CASGoogle Scholar
• 95. Penchovsky R. Computational design of allosteric ribozymes as molecular biosensors. Biotechnol. Adv. 32(5), 1015–1027 (2014).
  Medline CASGoogle Scholar
• 96. Perez-Herrero E, Fernandez-Medarde A. Advanced targeted therapies in cancer: drug nanocarriers, the future of chemotherapy. Eur. J. Pharm. Biopharm. 93, 52–79 (2015).
  Medline CASGoogle Scholar
• 97. Del Vecchio D, Dy AJ, Qian Y. Control theory meets synthetic biology. J. R. Soc. Interface 13(120), 1–17 (2016).
  Google Scholar
• 98. Khalil AS, Collins JJ. Synthetic biology: applications come of age. Nat. Rev. Genet. 11(5), 367–379 (2010).
  Medline CASGoogle Scholar
• 99. Lu TK, Collins JJ. Dispersing biofilms with engineered enzymatic bacteriophage. Proc. Natl Acad. Sci. USA 104(27), 11197–11202 (2007).
  Medline CASGoogle Scholar
• 100. Anderson JC, Clarke EJ, Arkin AP, Voigt CA. Environmentally controlled invasion of cancer cells by engineered bacteria. J. Mol. Biol. 355(4), 619–627 (2006).
  Medline CASGoogle Scholar
• 101. Krinsky N, Kaduri M, Zinger A et al. Synthetic cells synthesize therapeutic proteins inside tumors. Adv. Healthc. Mater. 7(9), e1701163 (2018).
  MedlineGoogle Scholar
• 102. Ghosh N, Kundu LM. Breaker peptides against amyloid-β aggregation: a potential therapeutic strategy for Alzheimer's disease. Future Med. Chem. 13(20), 1767–1794 (2021).
  CASGoogle Scholar
• 103. Ji L, Zhang F, Zhu L, Jiang J. An in-situ fabrication of bamboo bacterial cellulose/sodium alginate nanocomposite hydrogels as carrier materials for controlled protein drug delivery. Inter. J. Bio. Macromol. 170, 459–468 (2021).
  Medline CASGoogle Scholar
• 104. Majcher MJ, Babar A, Lofts A et al. In situ-gelling starch nanoparticle (SNP)/O-carboxymethyl chitosan (CMCh) nanoparticle network hydrogels for the intranasal delivery of an antipsychotic peptide. J. Control. Rel. 330, 738–752 (2021).
  Medline CASGoogle Scholar
• 105. Asgari M, Miri T, Soleymani M, Barati A. A novel method for in situ encapsulation of curcumin in magnetite-silica core-shell nanocomposites: a multifunctional platform for controlled drug delivery and magnetic hyperthermia therapy. J. Mol. Liquids 324, 114731 (2021).
  CASGoogle Scholar
• 106. Vigani B, Rossi S, Sandri G, Bonferoni MC, Caramella CM, Ferrari F. Recent advances in the development of in situ gelling drug delivery systems for non-parenteral administration routes. Pharmaceutics 12(9), 1–29 (2020).
  Google Scholar
• 107. Xie Y, Yang Y, He Y et al. Synthetic biology speeds up drug target discovery. Front. Pharmacol. 11, 119 (2020).
  Medline CASGoogle Scholar
• 108. Sercombe L, Veerati T, Moheimani F, Wu SY, Sood AK, Hua S. Advances and challenges of liposome assisted drug delivery. Front. Pharmacol. 6, 286 (2015).
  MedlineGoogle Scholar
• 109. Schwendener RA. Liposomes as vaccine delivery systems: a review of the recent advances. Ther. Adv. Vaccines 2(6), 159–182 (2014).
  Medline CASGoogle Scholar
• 110. Amidi M, de Raad M, Crommelin DJ, Hennink WE, Mastrobattista E. Antigen-expressing immunostimulatory liposomes as a genetically programmable synthetic vaccine. Syst. Synth. Biol. 5(1-2), 21–31 (2011).
  MedlineGoogle Scholar
• 111. Nissim L, Wu MR, Pery E et al. Synthetic RNA-based immunomodulatory gene circuits for cancer immunotherapy. Cell 171(5), 1138–1150 e1115 (2017).
  Medline CASGoogle Scholar
• 112. Thi Nhu Thao T, Labroussaa F, Ebert N et al. Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature 582(7813), 561–565 (2020).
  MedlineGoogle Scholar
• 113. Zhao W. A forum on synthetic biology: meet the great challenges with new technology. National Sci. Rev. 8(1), 1 (2020).
  Google Scholar
• 114. Zhang L-C, Zhao H-l, Liu J, He L, Yu R-l, Kang C-M. Design of SARS-CoV-2 Mpro, PLpro dual-target inhibitors based on deep reinforcement learning and virtual screening. Future Med. Chem. 14(6), 393–405 (2022).
  CASGoogle Scholar
• 115. Yang X, Duan J, Wu L. Research advances in NQO1-responsive prodrugs and nanocarriers for cancer treatment. Future Med. Chem. 14(5), 363–383 (2022).
  CASGoogle Scholar
• 116. Dong G, Ding Y, He S, Sheng C. Molecular glues for targeted protein degradation: from serendipity to rational discovery. J. Med. Chem. 64(15), 10606–10620 (2021).
  Medline CASGoogle Scholar
• 117. Li J, Zhao H, Zheng L, An W. Advances in synthetic biology and biosafety governance. Front. Bioeng. Biotechnol. 9, 598087 (2021).
  MedlineGoogle Scholar
• 118. Bernstein HS, Srivastava D. Stem cell therapy for cardiac disease. Pediatr. Res. 71(4 Pt 2), 491–499 (2012).
  Medline CASGoogle Scholar
• 119. Wong SS, Bernstein HS. Cardiac regeneration using human embryonic stem cells: producing cells for future therapy. Regen. Med. 5(5), 763–775 (2010).
  MedlineGoogle Scholar